Monitoring voltage-dependent charge displacement of Shaker B-IR K+ ion channels using radio frequency interrogation.

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Dharia, Sameera; Rabbitt, Richard D.;
(2011)
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE,volume 6,issue 2 (issn: 1932-6203, eissn: 1932-6203)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1371/journal.pone.0017363, pmc: PMC3046147
  • Subject: Biotechnology | Animal Models | Research Article | Molecular Cell Biology | Signal Processing | Signal Filtering | Membranes and Sorting | Proteins | Physics | Biophysics | Electronics Engineering | Electric Conductivity | Xenopus Laevis | Biology | Electrostatics | Neuroscience | Molecular Neuroscience | Protein Chemistry | Electricity | Electric Field | Medicine | Engineering | Anatomy and Physiology | Ion Channels | Bioengineering | Q | Biophysics Theory | R | Biological Systems Engineering | Electrophysiology | Model Organisms | Biochemistry | Science

Here we introduce a new technique that probes voltage-dependent charge displacements of excitable membrane-bound proteins using extracellularly applied radio frequency (RF, 500 kHz) electric fields. Xenopus oocytes were used as a model cell for these experiments, and we... View more