publication . Article . 2013

Osbpl8 deficiency in mouse causes an elevation of high-density lipoproteins and gender-specific alterations of lipid metabolism.

Eija Nissila; Mikko Mäyränpää; Vesa Olkkonen;
Open Access
  • Published: 15 Mar 2013 Journal: PLoS ONE, volume 8, page e58856 (eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
  • Country: Finland
Abstract
OSBP-related protein 8 (ORP8) encoded by Osbpl8 is an endoplasmic reticulum sterol sensor implicated in cellular lipid metabolism. We generated an Osbpl8(-/-) (KO) C57Bl/6 mouse strain. Wild-type and Osbpl8KO animals at the age of 13-weeks were fed for 5 weeks either chow or high-fat diet, and their plasma lipids/lipoproteins and hepatic lipids were analyzed. The chow-fed Osbpl8KO male mice showed a marked elevation of high-density lipoprotein (HDL) cholesterol (+79%) and phospholipids (+35%), while only minor increase of apolipoprotein A-I (apoA-I) was detected. In chow-fed female KO mice a less prominent increase of HDL cholesterol (+27%) was observed, while o...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Medicine, R, Science, Q, OXYSTEROL-BINDING PROTEINS, LECITHIN-CHOLESTEROL ACYLTRANSFERASE, PLASMA, ABCA1, HDL, ACTIVATION, EXPRESSION, STEROLS, FAMILY, SERUM, 3111 Biomedicine, 3121 Internal medicine, Research Article, Biology, Biochemistry, Lipids, Metabolism, Model Organisms, Animal Models, Mouse, Molecular Cell Biology, Anatomy and Physiology, Cardiovascular
47 references, page 1 of 4

1 Taylor FR, Saucier SE, Shown EP, Parish EJ, Kandutsch AA (1984) Correlation between oxysterol binding to a cytosolic binding protein and potency in the repression of hydroxymethylglutaryl coenzyme A reductase. J Biol Chem 259: 12382–12387.6490619 [PubMed]

2 Ridgway ND (2010) Oxysterol-binding proteins. Subcell Biochem 51: 159–182.20213544 [PubMed]

3 Perry RJ, Ridgway ND (2006) Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein. Mol Biol Cell 17: 2604–16.16571669 [OpenAIRE] [PubMed]

4 Wang PY, Weng J, Anderson RG (2005) OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science 307: 1472–6.15746430 [PubMed]

5 Lehto M, Laitinen S, Chinetti G, Johansson M, Ehnholm C, et al (2001) The OSBP-related protein family in humans. J Lipid Res 42: 1203–1213.11483621 [PubMed]

6 Jaworski CJ, Moreira E, Li A, Lee R, Rodriguez IR (2001) A family of 12 human genes containing oxysterol-binding domains. Genomics 78(3): 185–196.11735225 [OpenAIRE] [PubMed]

7 Anniss AM, Apostolopoulos J, Dworkin S, Purton LE, Sparrow RL (2002) An oxysterol-binding protein family identified in the mouse. DNA Cell Biol 21: 571–580.12215260 [OpenAIRE] [PubMed]

8 Raychaudhuri S, Prinz WA (2010) The diverse functions of oxysterol-binding proteins. Annu Rev Cell Dev Biol 26: 157–177.19575662 [OpenAIRE] [PubMed]

9 Vihervaara T, Jansen M, Uronen RL, Ohsaki Y, Ikonen E, et al (2011) Cytoplasmic oxysterol-binding proteins: Sterol sensors or transporters? Chem Ph ys Lipids 164: 443–450.21419754 [OpenAIRE] [PubMed]

10 Yan D, MäyränpääMI, Wong J, PerttiläJ, Lehto M, et al (2008) OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem 283: 332–340.17991739 [PubMed]

11 Vihervaara T, Käkelä R, Liebisch G, Tarasov K, Schmitz G, et al.. (2012) Modification of the lipidome in Raw264.7 macrophage subjected to stable silencing of oxysterol- binding proteins. Biochimie May 16. [Epub ahead of print] PMID: 22609461.

12 Zhou T, Li S, Zhong W, Vihervaara T, Beaslas O, et al (2011) OSBP-related protein 8 (ORP8) regulates plasma and liver tissue lipid levels and interacts with the nucleoporin Nup62. PLoS On e 6 e21078: 10.1371/journal.pone.0021078.

13 Jordan SD, Kruger M, Willmes DM, Redemann N, Wunderlich FT, et al (2011) Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 13: 434–446.21441927 [PubMed]

14 Calabresi L, Franceschini G (2010) Lecithin:Cholesterol acyltransferase, high-density lipoproteins, and atheroprotection in humans. Trends Cardiovasc Med 20: 50–53.20656215 [OpenAIRE] [PubMed]

15 Yazdanyar A, Yeang C, Jiang XC (2011) Role of phospholipid transfer protein in high-density lipoprotein- mediated reverse cholesterol transport. Curr Atheroscler Rep 13: 242–248.21365262 [OpenAIRE] [PubMed]

47 references, page 1 of 4
Abstract
OSBP-related protein 8 (ORP8) encoded by Osbpl8 is an endoplasmic reticulum sterol sensor implicated in cellular lipid metabolism. We generated an Osbpl8(-/-) (KO) C57Bl/6 mouse strain. Wild-type and Osbpl8KO animals at the age of 13-weeks were fed for 5 weeks either chow or high-fat diet, and their plasma lipids/lipoproteins and hepatic lipids were analyzed. The chow-fed Osbpl8KO male mice showed a marked elevation of high-density lipoprotein (HDL) cholesterol (+79%) and phospholipids (+35%), while only minor increase of apolipoprotein A-I (apoA-I) was detected. In chow-fed female KO mice a less prominent increase of HDL cholesterol (+27%) was observed, while o...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Medicine, R, Science, Q, OXYSTEROL-BINDING PROTEINS, LECITHIN-CHOLESTEROL ACYLTRANSFERASE, PLASMA, ABCA1, HDL, ACTIVATION, EXPRESSION, STEROLS, FAMILY, SERUM, 3111 Biomedicine, 3121 Internal medicine, Research Article, Biology, Biochemistry, Lipids, Metabolism, Model Organisms, Animal Models, Mouse, Molecular Cell Biology, Anatomy and Physiology, Cardiovascular
47 references, page 1 of 4

1 Taylor FR, Saucier SE, Shown EP, Parish EJ, Kandutsch AA (1984) Correlation between oxysterol binding to a cytosolic binding protein and potency in the repression of hydroxymethylglutaryl coenzyme A reductase. J Biol Chem 259: 12382–12387.6490619 [PubMed]

2 Ridgway ND (2010) Oxysterol-binding proteins. Subcell Biochem 51: 159–182.20213544 [PubMed]

3 Perry RJ, Ridgway ND (2006) Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein. Mol Biol Cell 17: 2604–16.16571669 [OpenAIRE] [PubMed]

4 Wang PY, Weng J, Anderson RG (2005) OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science 307: 1472–6.15746430 [PubMed]

5 Lehto M, Laitinen S, Chinetti G, Johansson M, Ehnholm C, et al (2001) The OSBP-related protein family in humans. J Lipid Res 42: 1203–1213.11483621 [PubMed]

6 Jaworski CJ, Moreira E, Li A, Lee R, Rodriguez IR (2001) A family of 12 human genes containing oxysterol-binding domains. Genomics 78(3): 185–196.11735225 [OpenAIRE] [PubMed]

7 Anniss AM, Apostolopoulos J, Dworkin S, Purton LE, Sparrow RL (2002) An oxysterol-binding protein family identified in the mouse. DNA Cell Biol 21: 571–580.12215260 [OpenAIRE] [PubMed]

8 Raychaudhuri S, Prinz WA (2010) The diverse functions of oxysterol-binding proteins. Annu Rev Cell Dev Biol 26: 157–177.19575662 [OpenAIRE] [PubMed]

9 Vihervaara T, Jansen M, Uronen RL, Ohsaki Y, Ikonen E, et al (2011) Cytoplasmic oxysterol-binding proteins: Sterol sensors or transporters? Chem Ph ys Lipids 164: 443–450.21419754 [OpenAIRE] [PubMed]

10 Yan D, MäyränpääMI, Wong J, PerttiläJ, Lehto M, et al (2008) OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem 283: 332–340.17991739 [PubMed]

11 Vihervaara T, Käkelä R, Liebisch G, Tarasov K, Schmitz G, et al.. (2012) Modification of the lipidome in Raw264.7 macrophage subjected to stable silencing of oxysterol- binding proteins. Biochimie May 16. [Epub ahead of print] PMID: 22609461.

12 Zhou T, Li S, Zhong W, Vihervaara T, Beaslas O, et al (2011) OSBP-related protein 8 (ORP8) regulates plasma and liver tissue lipid levels and interacts with the nucleoporin Nup62. PLoS On e 6 e21078: 10.1371/journal.pone.0021078.

13 Jordan SD, Kruger M, Willmes DM, Redemann N, Wunderlich FT, et al (2011) Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 13: 434–446.21441927 [PubMed]

14 Calabresi L, Franceschini G (2010) Lecithin:Cholesterol acyltransferase, high-density lipoproteins, and atheroprotection in humans. Trends Cardiovasc Med 20: 50–53.20656215 [OpenAIRE] [PubMed]

15 Yazdanyar A, Yeang C, Jiang XC (2011) Role of phospholipid transfer protein in high-density lipoprotein- mediated reverse cholesterol transport. Curr Atheroscler Rep 13: 242–248.21365262 [OpenAIRE] [PubMed]

47 references, page 1 of 4
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