Snapback Primer Mediated Clamping PCR for Detection of EGFR and KRAS Mutations in NSCLC Patients by High Resolution Melting Analysis

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Haiyan Sun; Yang Yang; Lixin Yang; Bo Su; Gening Jiang; Ke Fei; Daru Lu;
(2014)

Assays for detecting somatic mutations are requested with higher sensitivity and more convenience. Here, we describe snapback primer mediated allele clamping enrichment polymerase chain reaction (SPACE-PCR), a novel form of PCR that amplifies minority alleles selectivel... View more
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