Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action

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Rodrigues, Klinger Antonio da Franca ; Amorim, Layane Valéria ; Oliveira, Jamylla Mirck Guerra de ; Dias, Clarice Noleto ; Moraes, Denise Fernandes Coutinho ; Andrade, Eloisa Helena de Aguiar ; Maia, Jose Guilherme Soares ; Carneiro, Sabrina Maria Portela ; Carvalho, Fernando Aécio de Amorim (2013)
  • Publisher: Hindawi Publishing Corporation
  • Journal: Evidence-Based Complementary and Alternative Medicine, volume 2,013 (issn: 1741-427X, eissn: 1741-4288)
  • Related identifiers: doi: 10.1155/2013/279726, pmc: PMC3590759
  • Subject: Other systems of medicine | Research Article | RZ201-999 | Article Subject

Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%), γ-elemene (14.25%), and trans-β-elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μg·mL−1) and amastigotes (IC50, 1.92 μg·mL−1) suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μg·mL−1); however, there appeared to be no toxicity at 50 μg·mL−1. While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity.
  • References (60)
    60 references, page 1 of 6

    Kaur, S., Sachdeva, H., Dhuria, S., Sharma, M., Kaur, T.. Antileishmanial effect of cisplatin against murine visceral leishmaniasis. Parasitology International. 2010; 59 (1): 62-69

    Azzouz, S., Maache, M., Garcia, R. G., Osuna, A.. Leishmanicidal activity of edelfosine, miltefosine and ilmofosine. Basic and Clinical Pharmacology and Toxicology. 2005; 96 (1): 60-65

    Patza, J. A., Graczykb, T. K., Gellera, N., Vittorc, A. Y.. Effects of environmental change on emerging parasitic diseases. International Journal of Parasitology. 2000; 30 (12-13): 1295-1304

    Alizadeh, B. H., Foroumadi, A., Ardestani, S. K., Poorrajab, F., Shafiee, A.. Leishmanicidal evaluation of novel synthetic chromenes. Archiv der Pharmazie. 2008; 341 (12): 787-793

    Ashford, R. W.. The leishmaniases as emerging and reemerging zoonoses. International Journal for Parasitology. 2000; 30 (12-13): 1269-1281

    Rosa, M. S. S., Mendonça-Filho, R. R., Bizzo, H. R., Rodrigues, I. A., Soares, R. M., Souto-Padrón, T., Alviano, C. S., Lopes, A. H. C. S.. Antileishmanial activity of a linalool-rich essential oil from Croton cajucara. Antimicrobial Agents Chemotherapy. 2003; 47 (6): 1895-1901

    Ueda-Nakamura, T., Mendonça-Filho, R. R., Morgado-Díaz, J. A., Maza, P. K., Filho, B. P. D., Cortez, D. A. G., Alviano, D. S., Rosa, M. S. S., Lopes, A. H. C. S., Alviano, C. S., Nakamura, C. V.. Antileishmanial activity of eugenol-rich essential oil from Ocimum gratissimum. Parasitology International. 2006; 55 (2): 55-59

    Santos, A. O., Ueda-Nakamura, T., Dias Filho, B. P., Veiga Junior, V. F., Pinto, A. C., Nakamura, C. V.. Effect of Brazilian copaiba oils on Leishmania amazonensis. Journal of Ethnopharmacology. 2008; 120 (2): 204-208

    Monzote, L., Montalvo, A. M., Almanonni, S., Scull, R., Miranda, M., Abreu, J.. Activity of the essential oil from Chenopodium ambrosioides grown in Cuba against Leishmania amazonensis. Chemotherapy. 2006; 52 (3): 130-136

    Machado, M., Pires, P., Dinis, A. M., Santos-Rosa, M., Alves, V., Salgueiro, L., Cavaleiro, C., Sousa, M. C.. Monoterpenic aldehydes as potential anti-Leishmania agents: activity of Cymbopogon citratus and citral on L. infantum, L. tropica and L. major. Experimental Parasitology. 2012; 130 (3): 223-231

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