Characterization of plasma protein binding dissociation with online SPE-HPLC

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Ping Li ; Yiran Fan ; Yunlong Wang ; Yaxin Lu ; Zheng Yin (2015)
  • Publisher: Nature Publishing Group
  • Journal: Scientific Reports, volume 5 (issn: 2045-2322, eissn: 2045-2322)
  • Related identifiers: pmc: PMC4602217, doi: 10.1038/srep14866
  • Subject: Article
    mesheuropmc: viruses

A novel parameter of relative recovery (Rre) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of Rre with koff of PPB has been established with a new SPE model. A rapid, easy to use method could potentially be used to categorize PK properties of the drug candidates in the decision process of drug discovery and development.
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