Characterization of plasma protein binding dissociation with online SPE-HPLC
- Publisher: Nature Publishing Group
(issn: 2045-2322, eissn: 2045-2322)
A novel parameter of relative recovery (Rre) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of Rre with koff of PPB has been established with a new SPE model. A rapid, easy to use method could potentially be used to categorize PK properties of the drug candidates in the decision process of drug discovery and development.