publication . Article . 2000

Systemic administration of cationic phosphonolipids/DNA complexes and the relationship between formulation and lung transfection efficiency

Floch, V.; Delépine, P.; Guillaume, C.; Loisel, S.; Chassé, S.; Le Bolc’h, G.; Gobin, E.; Leroy, J.P.; Férec, C.;
Open Access English
  • Published: 01 Mar 2000 Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes, volume 1,464, issue 1, pages 95-103 (issn: 0005-2736, Copyright policy)
  • Publisher: Elsevier
Abstract
International audience; Performances of cationic lipid formulations for intravenous gene delivery to mouse lungs have been previously reported. We report in this study that cationic phosphonolipids, when appropriately formulated, can be good synthetic vectors for gene delivery to lung after intravenous administration. One of our reagents, GLB43, was capable of mediating a 500-fold higher expression in the lungs of mice than could be obtained with free pDNA alone (P=0.018). We demonstrate that the most important parameters for cationic phosphonolipid transfection activity after systemic administration are the chemical structure of the cationic phosphonolipid, the...
Subjects
free text keywords: Cell Biology, Cationic lipid, Gene transfer, Biochemistry, Formulation, [CHIM]Chemical Sciences, Intravenous administration, Biophysics
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publication . Article . 2000

Systemic administration of cationic phosphonolipids/DNA complexes and the relationship between formulation and lung transfection efficiency

Floch, V.; Delépine, P.; Guillaume, C.; Loisel, S.; Chassé, S.; Le Bolc’h, G.; Gobin, E.; Leroy, J.P.; Férec, C.;