Identification and validation of reference genes for qRT-PCR studies of the obligate aphid pathogenic fungus Pandora neoaphidis during different developmental stages.

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Zhang, Shutao; Chen, Chun; Xie, Tingna; Ye, Sudan;
(2017)
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE,volume 12,issue 7 (issn: 1932-6203, eissn: 1932-6203)
  • Publisher copyright policies & self-archiving
  • Related identifiers: pmc: PMC5495205, doi: 10.1371/journal.pone.0179930
  • Subject: Molecular Biology | Applied Mathematics | Algorithms | Microbial Pathogens | Research Article | Mathematics | Pathology and Laboratory Medicine | Cell Processes | Simulation and Modeling | Forms of DNA | Artificial Gene Amplification and Extension | Genetics | Physical Sciences | Pathogens | DNA | Molecular Biology Techniques | Fungal Pathogens | Polymerase Chain Reaction | Biology and Life Sciences | Research and Analysis Methods | Microbiology | Medicine | Nucleic acids | Fungal Genetics | Complementary DNA | Medical Microbiology | Q | R | Cell Biology | Fungi | Biochemistry | Science | Organisms | Medicine and Health Sciences | Mycology | Gene Expression | Cell Cycle and Cell Division

The selection of stable reference genes is a critical step for the accurate quantification of gene expression. To identify and validate the reference genes in Pandora neoaphidis–an obligate aphid pathogenic fungus—the expression of 13classical candidate reference genes ... View more
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