Derivation and characterization of Sleeping Beauty transposon-mediated porcine induced pluripotent stem cells

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Kues, Wilfried A.; Herrmann, Doris; Barg-Kues, Brigitte; Haridoss, Srividiameena; Nowak-Imialek, Monika; Buchholz, Thomas; Streeck, Miriam; Grebe, Antonia; Grabundzija, Ivana; Merkert, Sylvia; Martin, Ulrich; Hall, Vanessa J.; Rasmussen, Mikkel A.; Ivics, Zoltan; Hyttel, Poul; Niemann, Heiner;
  • Publisher: Mary Ann Liebert (U.S.A.)
  • Journal: volume 22, issue 1, pages 124-135issn: 1547-3287
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1089/scd.2012.0382
  • Subject: Differentiation | Transcriptome | Embryonic Stem Cells | SOXB1 Transcription Factors | Cell Transformation, Neoplastic | Mice | Antigens, Differentiation | Transgenes | Transposition | Porcine Pluripotent Cells | Fibroblasts | Cells, Cultured | Large Animal Model | Octamer Transcription Factor-3 | Microscopy, Fluorescence | Neurogenesis | Animals | Teratoma | 570 Life Sciences | Green Fluorescent Proteins | 610 Medical Sciences, Medicine | Sus scrofa | Coculture Techniques | DNA Transposable Elements | Cardiovascular and Metabolic Diseases | Mice, Nude | Promoter Regions, Genetic | Cell Imaging | Induced Pluripotent Stem Cells
    mesheuropmc: biological phenomena, cell phenomena, and immunity | embryonic structures | fungi

The domestic pig is an important large animal model for preclinical testing of novel cell therapies. Recently, we produced pluripotency reporter pigs in which the Oct4 promoter drives expression of the enhanced green fluorescent protein (EGFP). Here, we reprogrammed Oct... View more