Establishment of lipofection for studying miRNA function in human adipocytes.

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Eveliina Enlund ; Simon Fischer ; René Handrick ; Kerstin Otte ; Klaus-Michael Debatin ; Martin Wabitsch ; Pamela Fischer-Posovszky
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE, volume 9, issue 5 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: doi: 10.1371/journal.pone.0098023, pmc: PMC4029777
  • Subject: Lipids | Research Article | Biology and life sciences | Molecular Cell Biology | RNA | Synthetic Nucleic Acids | Medicine | Nucleic Acids | Epigenetics | Lipid Mediators | Q | R | Cell Biology | Genetics | Biochemistry | Science | RNA interference | Gene Expression

miRNA dysregulation has recently been linked to human obesity and its related complications such as type 2 diabetes. In order to study miRNA function in human adipocytes, we aimed for the modulation of mature miRNA concentration in these cells. Adipocytes, however, tend... View more
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