Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells
Hughes, Bryan G.
Hendzel, Michael J.
- Publisher: Elsevier
(issn: 2215-0161, eissn: 2215-0161)
Cell fractionation | Chemistry | Q | Membrane proteins | Sequential lysis | Science | Subcellular organelles | Nuclear proteins | Tissue fractionation
Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, th...