Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells

Article English OPEN
Baghirova, Sabina ; Hughes, Bryan G. ; Hendzel, Michael J. ; Schulz, Richard (2015)
  • Publisher: Elsevier
  • Journal: MethodsX, volume 2, pages 440-445 (issn: 2215-0161, eissn: 2215-0161)
  • Related identifiers: pmc: PMC4678309, doi: 10.1016/j.mex.2015.11.001
  • Subject: Cell fractionation | Chemistry | Q | Membrane proteins | Sequential lysis | Science | Subcellular organelles | Nuclear proteins | Tissue fractionation

Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, th... View more
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