Transcriptomic analysis of (group I) Clostridium botulinum ATCC 3502 cold shock response.

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Elias Dahlsten ; Marita Isokallio ; Panu Somervuo ; Miia Lindström ; Hannu Korkeala
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE, volume 9, issue 2 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: pmc: PMC3933689, doi: 10.1371/journal.pone.0089958
  • Subject: Microbial Control | Microbial Pathogens | Gram Positive | Research Article | Biology | Microbial Metabolism | Bacterial Physiology | Gastroenterology and Hepatology | Bacterial and Foodborne Illness | Infectious Diseases | Microbiology | Genome Expression Analysis | Medicine | Bacterial Pathogens | Microbial Ecology | Applied Microbiology | Bacterial Diseases | Q | Bacteriology | R | Microbial Physiology | Science | Genomics

Profound understanding of the mechanisms foodborne pathogenic bacteria utilize in adaptation to the environmental stress they encounter during food processing and storage is of paramount importance in design of control measures. Chill temperature is a central control measure applied in minimally processed foods; however, data on the mechanisms the foodborne pathogen Clostridium botulinum activates upon cold stress are scarce. Transcriptomic analysis on the C. botulinum ATCC 3502 strain upon temperature downshift from 37°C to 15°C was performed to identify the cold-responsive gene set of this organism. Significant up- or down-regulation of 16 and 11 genes, respectively, was observed 1 h after the cold shock. At 5 h after the temperature downshift, 199 and 210 genes were up- or down-regulated, respectively. Thus, the relatively small gene set affected initially indicated a targeted acute response to cold shock, whereas extensive metabolic remodeling appeared to take place after prolonged exposure to cold. Genes related to fatty acid biosynthesis, oxidative stress response, and iron uptake and storage were induced, in addition to mechanisms previously characterized as cold-tolerance related in bacteria. Furthermore, several uncharacterized DNA-binding transcriptional regulator-encoding genes were induced, suggesting involvement of novel regulatory mechanisms in the cold shock response of C. botulinum. The role of such regulators, CBO0477 and CBO0558A, in cold tolerance of C. botulinum ATCC 3502 was demonstrated by deteriorated growth of related mutants at 17°C.
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