A Novel Antimicrobial Coating Represses Biofilm and Virulence-Related Genes in Methicillin-Resistant Staphylococcus aureus

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Vaishampayan, Ankita ; de Jong, Anne ; Wight, Darren J. ; Kok, Jan ; Grohmann, Elisabeth (2018)
  • Publisher: Frontiers Media S.A.
  • Journal: Frontiers in Microbiology, volume 9 (eissn: 1664-302X)
  • Related identifiers: doi: 10.3389/fmicb.2018.00221, pmc: PMC5818464
  • Subject: MRSA | 616 Krankheiten | quorum-sensing | Microbiology | 614 Incidence and prevention of disease | antimicrobial surface | RNA sequencing | virulence | 616 Diseases | 579 Microorganisms, fungi, algae | biofilm | Original Research | 579 Mikroorganismen, Pilze und Algen (Fungi und Algae) | 614 Inzidenz und Prävention von Krankheiten
    • ddc: ddc:614 | ddc:579 | ddc:616
    mesheuropmc: biochemical phenomena, metabolism, and nutrition

Methicillin-resistant Staphylococcus aureus (MRSA) has become an important cause of hospital-acquired infections worldwide. It is one of the most threatening pathogens due to its multi-drug resistance and strong biofilm-forming capacity. Thus, there is an urgent need for novel alternative strategies to combat bacterial infections. Recently, we demonstrated that a novel antimicrobial surface coating, AGXX((R)), consisting of microgalvanic elements of the two noble metals, silver and ruthenium, surface-conditioned with ascorbic acid, efficiently inhibits MRSA growth. In this study, we demonstrated that the antimicrobial coating caused a significant reduction in biofilm formation (46%) of the clinical MRSA isolate, S. aureus 04-02981. To understand the molecular mechanism of the antimicrobial coating, we exposed S. aureus 04-02981 for different time-periods to the coating and investigated its molecular response via next-generation RNA-sequencing. A conventional antimicrobial silver coating served as a control. RNA-sequencing demonstrated down-regulation of many biofilm-associated genes and of genes related to virulence of S. aureus. The antimicrobial substance also down-regulated the two-component quorum-sensing system agr suggesting that it might interfere with quorum-sensing while diminishing biofilm formation in S. aureus 04-02981.
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