Identifying differences in mRNA expression by representational difference analysis of cDNA.
Article, Other literature type
Schatz, D. G.
Animals, B-Lymphocytes, Base Sequence, Caffeine, Cloning, Molecular, DNA, Complementary, Gene Expression, Gene Rearrangement, B-Lymphocyte, Genes, RAG-1, In Vitro Techniques, Mice, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction
Detection of differentially regulated genes has been severely hampered by technical limitations. In an effort to overcome these problems, the PCR-coupled subtractive process of representational difference analysis (RDA) [Lisitsyn, N. et al. (1993) Science 259, 946-951] ...