Application of infrared spectroscopy for diagnosis of kidney tumor tissue

Bachelor thesis Lithuanian OPEN
Bandzevičiūtė, Rimantė (2016)
  • Publisher: Institutional Repository of Vilnius University

Application of Infrared Spectroscopy for Diagnosis of Kidney Tumor Tissue It is possible to apply the technique of an attenuated total reflection of infrared radiation (ATR IR) for the characterisation of the removed tissues during the surgery. Application of this method for interstitium of the removed tissue does not require any specific sample preparation. For this reason ATR IR technique applied for the interstitium allows to get information about tissues immediately after surgical operation. The aim of this work is to register the ATR IR absorption spectra of interstitium of the human kidney tissue immediately after surgical operation and determine the differences between spectra of normal and tumor kidney tissue which could be used for identification of normal and tumor tissue and evaluation of the malignancy grade. The goals of this study: 1. To do the review of the literature. 2. To master the principles of the FT-IR spectroscopy. 3. To register the ATR IR absorption spectra of interstitium of the human kidney tissue. 4. To analyse the results and determine the differences between spectra of normal and tumor kidney tissue which could be used for identification of normal and tumor tissue and evaluation of the malignancy grade. Main results and conclusions. The primary results of the spectral analysis of the spectra of interstitium of normal and tumor kidney tissue showed that the main differences between the spectra of interstitium of normal and tumor tissue are in the area between 1200 cm-1 and 1000 cm-1 where the three bands of the glycogen are seen at 1156 cm-1, 1085 cm-1 and 1035 cm-1 in the tumor tissue spectra. The higher the malignancy grade is, the more intensive these bands are due to the fact that the tumor cells need more energy and they are tend to accumulate the glycogen as a source of energy. It could be said that the chemical composition of the tissue is not changed among different malignancy grades G1, G2 and G3 because the position of the absorption bands in the spectra are independent of the malignancy grade. The only change is the intensity of the bands which shows the changed amount of chemical components in the tissue. The present method of the experiment is suitable for the research because there are not any essential alterations in the spectra of the same tissue sample when the spectra are registered at different time moments.
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