publication . Article . 2012

Post-implantation mortality of in vitro produced embryos is associated with DNA methyltransferase 1 dysfunction in sheep placenta

PAOLA TOSCHI;
Closed Access English
  • Published: 20 Nov 2012 Journal: HUMAN REPRODUCTION (issn: 0268-1161, Copyright policy)
  • Publisher: OXFORD UNIV PRESS
Abstract
materials, setting, methods: Immediately after collection, early placental tissues (chorion-allantois) were snap frozen in liquid nitrogen and DNMT1 expression and activity was evaluated. mRNA levels (for DNMT1, HDAC2, PCNA, DMAP1, MEST, IGF2, CDKN1C, H19) and the methylation status of H19 were also analyzed. Furthermore, embryo size and survival rate were measured. main results and the role of chance: Our study shows that DNMT1 expression was reduced in early placentae from sheep IVP embryos. This reduction was associated with growth arrest and subsequent death of the sheep embryos. Conversely, normal levels of DNMT1 and its cofactors were observed in placentae...
Subjects
Medical Subject Headings: embryonic structuresanimal structures
free text keywords: Obstetrics and Gynaecology, Rehabilitation, Reproductive Medicine, Andrology, Embryo, Endocrinology, medicine.medical_specialty, medicine, Methylation, Genetics, Fetus, DNMT1, Internal medicine, Placenta, medicine.anatomical_structure, Proliferating cell nuclear antigen, biology.protein, biology, DNA methylation, DNA methyltransferase
Funded by
EC| NEXTGEN
Project
NEXTGEN
Next generation methods to preserve farm animal biodiversity by optimizing present and future breeding options
  • Funder: European Commission (EC)
  • Project Code: 244356
  • Funding stream: FP7 | SP1 | KBBE
,
EC| ANGIOPLACE
Project
ANGIOPLACE
Expression and Methylation Status of Genes Regulating Placental Angiogenesis in Normal, Cloned, IVF and Monoparental Sheep Foetuses
  • Funder: European Commission (EC)
  • Project Code: 210103
  • Funding stream: FP7 | SP2 | ERC
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