Antibacterial and antioxidant activities of Vaccinium corymbosum L. leaf extract
Other literature type, Article
Md Abul Hasnat
Beong Ou Lim
- Publisher: Asian Pacific Journal of Tropical Disease Editorial Office
Asian Pacific Journal of Tropical Disease
(issn: 2222-1808, eissn: 2222-1808)
Antioxidant | Free radicals | R | RC955-962 | Basic Researches | Vaccinium corymbosum | DNA damage | Phenolic compound | Antibacterial | Medicine | Flavonoid | Arctic medicine. Tropical medicine
Objective: To investigate antibacterial and antioxidant activity of the leaf extract of tropical
medicinal herb and food plant Vaccinium corymbosum L. (V. corymbosum) .
Methods: Free radical scavenging activity on DPPH, ABTS, and nitrites were used to analyse
phenolic and flavonoid contents of leaf extract. Other focuses included the determination of
antioxidant enzymatic activity (SOD, CAT and GPx), metal chelating activity, reduction power, lipid
peroxidation inhibition and the prevention of oxidative DNA damage. Antibacterial activity was
determined by using disc diffusion method against seven strains of bacteria.
Results: Results found that V. corymbosum leaf extract had significant antibacterial activity. The
tested extract displayed the highest activity (about 23.18 mm inhibition zone) against Salmonella
typhymurium and the lowest antibacterial activity was observed against Enterococcus faecalis
(about 14.08 mm inhibition zone) at 10 mg/ disc. The IC 50 values for DPPH, ABTS and radical
scavenging activity were 0.120, 0.049 and 1.160 mg/mL, respectively. V. corymbosum leaf extract
also showed dose dependent reduction power, lipid peroxidation, DNA damage prevention and
significant antioxidant enzymatic activity.
Conclusions: These findings demonstrate that leaf extract of V. corymbosum could be used as an
alternative therapy for antibiotic-resistant bacteria and help prevent various free radical related