Molecular Characterization of Three Lactobacillus delbrueckii subsp. bulgaricus Phages

Article, Other literature type English OPEN
Casey, Eoghan ; Mahony, Jennifer ; O'Connell-Motherway, Mary ; Bottacini, Francesca ; Cornelissen, Anneleen ; Neve, Horst ; Heller, Knut J. ; Noben, Jean-Paul ; Dal Bello, Fabio ; van Sinderen, Douwe (2014)
  • Publisher: American Society for Microbiology
  • Related identifiers: doi: 10.1128/AEM.01268-14
  • Subject: Genetics and Molecular Biology

In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations. E.C. is the recipient of a scholarship from the Irish Research Council Enterprise Partnership Scheme. D.V.S. was supported by a Principal Investigator award (reference numbers 08/IN.1/B1909 and 13/IA/1953) through Science Foundation Ireland (SFI). F.B. is supported by SFI under grants 07/CE/B1368 and SFI/12/RC/2273. A.C. is the recipient of an EMPOWER postdoctoral fellowship of the Irish Research Council. M.O.-M. is a recipient of an HRB postdoctoral fellowship (PDTM/20011/9). The Orbitrap mass spectrometric services provided in this study were supported by Hercules Foundation (Belgium) project R-3986 with technical assistance from Erik Royackers.
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