IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

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Pauly, Diana; Chacana, Pablo A.; Calzado, Esteban G.; Brembs, Björn; Schade, Rüdiger;
(2011)
  • Publisher: MyJove Corporation
  • Journal: Journal of Visualized Experiments : JoVE,issue 51 (eissn: 1940-087X)
  • Related identifiers: doi: 10.3791/3084, pmc: PMC3197133
  • Subject: Egg Yolk/immunology | Polyethylene Glycol | Chicken | Immunoglobulins/isolation & purification | Issue 51 | Immunoprecipitation/methods | Antibodies/chemistry | Chickens | Medizin | Antibodies/isolation & purification | Egg Yolk/chemistry | Immunization | Antibodies/immunology | Polyethylene Glycols/chemistry | Antibodies | Animals | Female | Chick Embryo | Immunoglobulins/chemistry | Immunology | Immunoglobulins/immunology
    • ddc: ddc:610

Hens can be immunized by means of i.m. vaccination (Musculus pectoralis, left and right, injection volume 0.5-1.0 ml) or by means of Gene-Gun plasmid-immunization. Dependent on the immunogenicity of the antigen, high antibody-titres (up to 1:100,000 - 1:1,000,000) can ... View more
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