Glass bead transformation method for gram-positive bacteria

Article English OPEN
Pongsak Rattanachaikunsopon; Parichat Phumkhachorn; (2009)
  • Publisher: Sociedade Brasileira de Microbiologia
  • Journal: Brazilian Journal of Microbiology,volume 40,issue 4,pages923-926 (issn: 1517-8382, eissn: 1678-4405)
  • Related identifiers: doi: 10.1590/S1517-83822009000400025, doi: 10.1590/S1517-838220090004000025, pmc: PMC3768579
  • Subject: Gram-positive bacteria | glass bead | General Microbiology | Microbiology | QR1-502 | transformation | Research Paper
    mesheuropmc: food and beverages | biochemical phenomena, metabolism, and nutrition | fungi | mental disorders

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into pr... View more
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