publication . Article . 2015

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Guo, Bingfu; Guo, Yong; Hong, Huilong; Jin, Longguo; Zhang, Lijuan; Chang, Ru-Zhen; Lu, Wei; Lin, Min; Qiu, Li-Juan;
Open Access English
  • Published: 15 Oct 2015 Journal: Frontiers in Plant Science, volume 6 (issn: 1664-462X, eissn: 1664-462X, Copyright policy)
  • Publisher: Frontiers Media S.A.
Abstract
Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tole...
Subjects
mesheuropmc: food and beverages
free text keywords: glyphosate tolerance, transgenic soybean, G2-EPSPS, Plant Science, Original Research, GAT, chlorophyll content, shikimic acid
51 references, page 1 of 4

Bradford M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 10.1016/0003-2697(76)90527-3 942051 [PubMed] [DOI]

Buehring N. W.Massey J. H.Reynolds D. B. (2007). Shikimic acid accumulation in field grown corn (Zea mays) following simulated glyphosate drift. J. Agric. Food Chem. 55, 819–824. 10.1021/jf062624f 17263480 [PubMed] [DOI]

Campbell W. F.Evans J. O.Reed S. C. (1976). Effect of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci. 24, 22–25.

Castle L. A.Daniel L. S.Gorton R.Patten P. A.Chen Y. H.Bertain S. (2004). Discovery and directed evolution of a glyphosate tolerance gene. Science 304, 1151–1154. 10.1126/science.1096770 15155947 [PubMed] [DOI]

Dill G. M.CaJacob C. A.Padgette S. R. (2008). Glyphosate-resistant crops: adoption, use and future considerations. Pest Manag. Sci. 64, 326–331. 10.1002/ps.1501 18078304 [PubMed] [DOI]

Ding W.Reddy K. N.Zablotowicz R. M.Bellaloui N.Bruns H. A. (2011). Physiological responses of glyphosate-resistant and glyphosate-sensitive soybean to aminomethylphosphonic acid, a metabolite of glyphosate. Chemosphere 83, 593–598. 10.1016/j.chemosphere.2010.12.008 21190714 [PubMed] [DOI]

Dun B. Q.Wang X. J.Lu W.Chen M.Zhang W.Ping S. Z. (2014). Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes. Crop J. 2, 164–169. 10.1016/j.cj.2014.03.003 [OpenAIRE] [DOI]

Dun B. Q.Wang X. J.Lu W.Zhao Z. L.Hou S. N.Zhang B. M. (2007). Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco. Appl. Environ. Microbiol. 73, 7997–8000. 10.1128/AEM.00956-07 17951442 [OpenAIRE] [PubMed] [DOI]

Edwards R.Hannah M. (2014). Focus on weed control. Plant Physiol. 166, 1087–1089. 10.1104/pp.114.900496 25378696 [OpenAIRE] [PubMed] [DOI]

Escorial M. C.Sixto H.Gracia-Baudin J. M.Chueca M. C. (2001). A rapid method to determine cereal plant response to glyphosate. Weed Technol. 15, 697–702. 10.1614/0890-037X(2001)015[0697:ARMTDC]2.0.CO;2 [DOI]

Franz J. E.Mao M. K.Sikorski J. A. (1997). Glyphosate: a Unique Global Herbicide. Washington, DC: American Chemical Society.

Gazziero D. L. P.Adegas F.Voll E. (2008). Glifosate e Soja Transgênica. Circular Técnica 60. Londrina: Embrapa Soja, p4.

Gao Y.Liu H.Tao B. (2013). Screening for glyphosate resistant wild soybean (Glycine soja) and study in its physiological mechanisms of resistance. Agric. Sci. Technol. 14, 1263–1266.

Green J. M. (2012). The benefits of herbicide-resistant crops. Pest Manag. Sci. 68, 1323–1331. 10.1002/ps.3374 22865693 [PubMed] [DOI]

Green J. M.Hazel C. B.Forney D. R.Pugh L. M. (2008). New multiple-herbicide crop resistance and formulation technology to augment the utility of glyphosate. Pest Manag. Sci. 64, 332–339. 10.1002/ps.1486 18069651 [PubMed] [DOI]

51 references, page 1 of 4
Abstract
Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tole...
Subjects
mesheuropmc: food and beverages
free text keywords: glyphosate tolerance, transgenic soybean, G2-EPSPS, Plant Science, Original Research, GAT, chlorophyll content, shikimic acid
51 references, page 1 of 4

Bradford M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 10.1016/0003-2697(76)90527-3 942051 [PubMed] [DOI]

Buehring N. W.Massey J. H.Reynolds D. B. (2007). Shikimic acid accumulation in field grown corn (Zea mays) following simulated glyphosate drift. J. Agric. Food Chem. 55, 819–824. 10.1021/jf062624f 17263480 [PubMed] [DOI]

Campbell W. F.Evans J. O.Reed S. C. (1976). Effect of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci. 24, 22–25.

Castle L. A.Daniel L. S.Gorton R.Patten P. A.Chen Y. H.Bertain S. (2004). Discovery and directed evolution of a glyphosate tolerance gene. Science 304, 1151–1154. 10.1126/science.1096770 15155947 [PubMed] [DOI]

Dill G. M.CaJacob C. A.Padgette S. R. (2008). Glyphosate-resistant crops: adoption, use and future considerations. Pest Manag. Sci. 64, 326–331. 10.1002/ps.1501 18078304 [PubMed] [DOI]

Ding W.Reddy K. N.Zablotowicz R. M.Bellaloui N.Bruns H. A. (2011). Physiological responses of glyphosate-resistant and glyphosate-sensitive soybean to aminomethylphosphonic acid, a metabolite of glyphosate. Chemosphere 83, 593–598. 10.1016/j.chemosphere.2010.12.008 21190714 [PubMed] [DOI]

Dun B. Q.Wang X. J.Lu W.Chen M.Zhang W.Ping S. Z. (2014). Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes. Crop J. 2, 164–169. 10.1016/j.cj.2014.03.003 [OpenAIRE] [DOI]

Dun B. Q.Wang X. J.Lu W.Zhao Z. L.Hou S. N.Zhang B. M. (2007). Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco. Appl. Environ. Microbiol. 73, 7997–8000. 10.1128/AEM.00956-07 17951442 [OpenAIRE] [PubMed] [DOI]

Edwards R.Hannah M. (2014). Focus on weed control. Plant Physiol. 166, 1087–1089. 10.1104/pp.114.900496 25378696 [OpenAIRE] [PubMed] [DOI]

Escorial M. C.Sixto H.Gracia-Baudin J. M.Chueca M. C. (2001). A rapid method to determine cereal plant response to glyphosate. Weed Technol. 15, 697–702. 10.1614/0890-037X(2001)015[0697:ARMTDC]2.0.CO;2 [DOI]

Franz J. E.Mao M. K.Sikorski J. A. (1997). Glyphosate: a Unique Global Herbicide. Washington, DC: American Chemical Society.

Gazziero D. L. P.Adegas F.Voll E. (2008). Glifosate e Soja Transgênica. Circular Técnica 60. Londrina: Embrapa Soja, p4.

Gao Y.Liu H.Tao B. (2013). Screening for glyphosate resistant wild soybean (Glycine soja) and study in its physiological mechanisms of resistance. Agric. Sci. Technol. 14, 1263–1266.

Green J. M. (2012). The benefits of herbicide-resistant crops. Pest Manag. Sci. 68, 1323–1331. 10.1002/ps.3374 22865693 [PubMed] [DOI]

Green J. M.Hazel C. B.Forney D. R.Pugh L. M. (2008). New multiple-herbicide crop resistance and formulation technology to augment the utility of glyphosate. Pest Manag. Sci. 64, 332–339. 10.1002/ps.1486 18069651 [PubMed] [DOI]

51 references, page 1 of 4
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . 2015

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Guo, Bingfu; Guo, Yong; Hong, Huilong; Jin, Longguo; Zhang, Lijuan; Chang, Ru-Zhen; Lu, Wei; Lin, Min; Qiu, Li-Juan;