Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies

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López-Matas, M. Angeles ; Gallego, Mayte ; Iraola, Víctor ; Robinson, Douglas ; Carnés, Jerónimo (2013)
  • Publisher: Hindawi Publishing Corporation
  • Journal: BioMed Research International, volume 2,013 (issn: 2314-6133, eissn: 2314-6141)
  • Related identifiers: doi: 10.1155/2013/284615, pmc: PMC3816019
  • Subject: R | Research Article | Medicine | Article Subject
    mesheuropmc: chemical and pharmacologic phenomena

Background. The synthesis of allergen-specific blocking IgGs that interact with IgE after allergen immunotherapy (SIT) has been related to clinical efficacy. The objectives were to investigate the epitope specificity of IgG-antibodies induced by depigmented-polymerized (Dpg-Pol) allergoids and unmodified allergen extracts, and examine IgE-blocking activity of induced IgG-antibodies. Methods. Rabbits were immunized with native and Dpg-Pol extracts of birch pollen, and serum samples were obtained. Recognition of linear IgG-epitopes of Bet v 1 and Bet v 2 and the capacity of these IgG-antibodies to block binding of human-IgE was determined. Results. Serum from rabbits immunized with native extracts recognised 11 linear epitopes from Bet v 1, while that from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from native immunized animals, and 9 from Dpg-Pol immunized one. Dpg-Pol and native immunized serum did not always recognise the same epitopes, but specific-IgG from both could block human-IgE binding sites for native extract. Conclusions. Depigmented-polymerized birch extract stimulates the synthesis of specific IgG-antibodies which recognize common but also novel epitopes compared with native extracts. IgG-antibodies induced by Dpg-Pol effectively inhibit human-IgE binding to allergens which may be part of the mechanism of action of SIT.
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