Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA

Article English OPEN
Wang, Hongyang ; Owens, James D ; Shih, Joanna H ; Li, Ming-Chung ; Bonner, Robert F ; Mushinski, J Frederic (2006)
  • Publisher: BioMed Central
  • Journal: BMC Genomics, volume 7, pages 97-97 (issn: 1471-2164, eissn: 1471-2164)
  • Related identifiers: pmc: PMC1513394, doi: 10.1186/1471-2164-7-97
  • Subject: Biotechnology | QH426-470 | Genetics | Research Article | TP248.13-248.65

<p>Abstract</p> <p>Background</p> <p>Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with fro... View more
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