Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

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Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir, Ankica ; Vukičević, Slobodan ; Fulgosi, Hrvoje (2015)
  • Publisher: Elsevier BV
  • Journal: Biotechnology Reports, volume 7, pages 81-86 (issn: 2215-017X, eissn: 2215-017X)
  • Related identifiers: doi: 10.1016/j.btre.2015.05.006, pmc: PMC5466047
  • Subject: Vacuum infiltration | Transient expression | GM-CSF | Tobacco | Heterologous expression | Article
    mesheuropmc: food and beverages

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.
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