Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis

Article English OPEN
Xian-Quan Cai ; Hai-Qiong Yu ; Rong Li ; Qiao-Yun Yue ; Guo-Hua Liu ; Jian-Shan Bai ; Yan Deng ; De-Yi Qiu ; Xing-Quan Zhu (2014)
  • Publisher: Hindawi Limited
  • Journal: The Scientific World Journal, volume 2,014 (issn: 2356-6140, eissn: 1537-744X)
  • Related identifiers: pmc: PMC4217323, doi: 10.1155/2014/893981
  • Subject: Research Article | Science (General) | Q1-390 | Article Subject
    mesheuropmc: fungi | parasitic diseases

Clonorchis sinensis and Opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in Asia. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the specific detection an... View more
  • References (20)
    20 references, page 1 of 2

    Fried, B., Reddy, A., Mayer, D.. Helminths in human carcinogenesis. Cancer Letters. 2011; 305 (2): 239-249

    Xue-Ming, L., Ying-Dan, C., Ouyang, Y., Hong-Man, Z., Rui, L., Wei, M.. Overview of human clonorchiasis sinensis in China. Southeast Asian Journal of Tropical Medicine and Public Health. 2011; 42 (2): 248-254

    Sripa, B., Kaewkes, S., Intapan, P. M., Maleewong, W., Brindley, P. J.. Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control. Advances in Parasitology. 2010; 72: 305-350

    Hong, S., Fang, Y.. Clonorchis sinensis and clonorchiasis, an update. Parasitology International. 2012; 61 (1): 17-24

    Young, N. D., Campbell, B. E., Hall, R. S., Jex, A. R., Cantacessi, C., Laha, T., Sohn, W., Sripa, B., Loukas, A., Brindley, P. J., Gasser, R. B.. Unlocking the transcriptomes of two carcinogenic parasites, Clonorchis sinensis and Opisthorchis viverrini. PLoS Neglected Tropical Diseases. 2010; 4 (6, article e719)

    Marcos, L. A., Terashima, A., Gotuzzo, E.. Update on hepatobiliary flukes: fascioliasis, opisthorchiasis and clonorchiasis. Current Opinion in Infectious Diseases. 2008; 21 (5): 523-530

    Sirisinha, S., Sahassananda, D., Bunnag, D., Rim, H. J.. Immunological analysis of Opisthorchis and Clonorchis antigens. Journal of Helminthology. 1990; 64 (2): 133-138

    Traub, R. J., Macaranas, J., Mungthin, M., Leelayoova, S., Cribb, T., Murrell, K. D., Thompson, R. C. A.. A new PCR-based approach indicates the range of Clonorchis sinensis now extends to Central Thailand. PLoS Neglected Tropical Diseases. 2009; 3 (1, article e367)

    Cai, X. Q., Xu, M. J., Wang, Y. H., Qiu, D. Y., Liu, G. X., Lin, A., Tang, J. D., Zhang, R. L., Zhu, X. Q.. Sensitive and rapid detection of Clonorchis sinensis infection in fish by loop-mediated isothermal amplification (LAMP). Parasitology Research. 2010; 106 (6): 1379-1383

    Schuster, R. K.. Opisthorchiidosis—a review. Infectious Disorders—Drug Targets. 2010; 10 (5): 402-415

  • Related Research Results (1)
  • Metrics
    No metrics available
Share - Bookmark