Alteration of Sequence Specificity of the Type IIS Restriction Endonuclease BtsI

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Shengxi Guan; Aine Blanchard; Penghua Zhang; Zhenyu Zhu;

The Type IIS restriction endonuclease BtsI recognizes and digests at GCAGTG(2/0). It comprises two subunits: BtsIA and BtsIB. The BtsIB subunit contains the recognition domain, one catalytic domain for bottom strand nicking and part of the catalytic domain for the top s... View more
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