publication . Article . 2017

A salivary EF-hand calcium-binding protein of the brown planthopper Nilaparvata lugens functions as an effector for defense responses in rice

Ye, Wenfeng; Yu, Haixin; Jian, Yukun; Zeng, Jiamei; Ji, Rui; Chen, Hongdan; Lou, Yonggen;
Open Access English
  • Published: 18 Jan 2017 Journal: Scientific Reports, volume 7 (issn: 2045-2322, eissn: 2045-2322, Copyright policy)
  • Publisher: Nature Publishing Group
Abstract
The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), a major pest of rice in Asia, is able to successfully puncture sieve tubes in rice with its piercing stylet and then to ingest phloem sap. How BPH manages to continuously feed on rice remains unclear. Here, we cloned the gene NlSEF1, which is highly expressed in the salivary glands of BPH. The NlSEF1 protein has EF-hand Ca2+-binding activity and can be secreted into rice plants when BPH feed. Infestation of rice by BPH nymphs whose NlSEF1 was knocked down elicited higher levels of Ca2+ and H2O2 but not jasmonic acid, jasmonoyl-isoleucine (JA-Ile) and SA in rice than did infestation ...
Subjects
mesheuropmc: food and beveragesurologic and male genital diseasesurogenital system
free text keywords: Article
50 references, page 1 of 4

Mithoefer A. & Boland W. Plant defense against herbivores: chemical aspects. Annu Rev Plant Biol 63, 431–450 (2012).22404468 [PubMed]

Wu J. & Baldwin I. T. New insights into plant responses to the attack from insect herbivores. Annu Rev Genet 44, 1–24 (2010).20649 414 [PubMed]

Hogenhout S. A. & Bos J. I. B. Effector proteins that modulate plant-insect interactions. Curr Opin Plant Biol 14, 422–428 (2011).21684190 [PubMed]

Erb M., Meldau S. & Howe G. A. Role of phytohormones in insect-specific plant reactions. Trends Plant Sci 17, 250–259 (2012).22305233 [OpenAIRE] [PubMed]

Schaefer M.. Lipase activity in insect oral secretions mediates defense responses in Arabidopsis. Plant Physiol 156, 1520–1534 (2011).21546453 [OpenAIRE] [PubMed]

Sharma A.. Salivary proteins of plant-feeding hemipteroids-implication in phytophagy. B Entomol Res 104, 117–136 (2014).

Atamian H. S.. In planta expression or delivery of potato aphid Macrosiphum euphorbiae effectors Me10 and Me23 enhances aphid fecundity. Mol Plant Microbe Interact 26, 67–74 (2013).23194342 [PubMed]

Will T., Tjallingii W. F., Thonnessen A. & van Bel A. J. Molecular sabotage of plant defense by aphid saliva. Proc Natl Acad Sci USA 104, 10536–10541 (2007).17553961 [OpenAIRE] [PubMed]

Hattori M.. Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper Nephotettix cincticeps. Insect Biochem Mol Biol 42, 1–9 (2012).22019571 [PubMed]

Consales F.. Insect oral secretions suppress wound-induced responses in Arabidopsis. J EXP Bot 63, 727–737 (2012).21994175 [OpenAIRE] [PubMed]

Steinhorst L. & Kudla J. Signaling in cells and organisms-calcium holds the line. Curr Opin Plant Biol 22, 14–21 (2014).25195171 [PubMed]

Kudla J., Batistic O. & Hashimoto K. Calcium signals: the lead currency of plant information processing. Plant Cell 22, 541–563 (2010).20354197 [OpenAIRE] [PubMed]

Grabarek Z.Insights into modulation of calcium signaling by magnesium in calmodulin, troponin C and related EF-hand proteins. Biochim Biophys Acta 1813, 913–921 (2011).21262274 [OpenAIRE] [PubMed]

Poovaiah B. W., Du L., Wang H. & Yang T. Recent advances in calcium/calmodulin-mediated signaling with an em phasis on plant-microbe interactions. Plant Physiol 163, 531–542 (2013).24014576 [OpenAIRE] [PubMed]

Batistic O. & Kudla J. Analysis of calcium signaling pathways in plants. Biochim Biophys Acta 1820, 1283–1293 (2012).22061997 [PubMed]

50 references, page 1 of 4
Abstract
The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), a major pest of rice in Asia, is able to successfully puncture sieve tubes in rice with its piercing stylet and then to ingest phloem sap. How BPH manages to continuously feed on rice remains unclear. Here, we cloned the gene NlSEF1, which is highly expressed in the salivary glands of BPH. The NlSEF1 protein has EF-hand Ca2+-binding activity and can be secreted into rice plants when BPH feed. Infestation of rice by BPH nymphs whose NlSEF1 was knocked down elicited higher levels of Ca2+ and H2O2 but not jasmonic acid, jasmonoyl-isoleucine (JA-Ile) and SA in rice than did infestation ...
Subjects
mesheuropmc: food and beveragesurologic and male genital diseasesurogenital system
free text keywords: Article
50 references, page 1 of 4

Mithoefer A. & Boland W. Plant defense against herbivores: chemical aspects. Annu Rev Plant Biol 63, 431–450 (2012).22404468 [PubMed]

Wu J. & Baldwin I. T. New insights into plant responses to the attack from insect herbivores. Annu Rev Genet 44, 1–24 (2010).20649 414 [PubMed]

Hogenhout S. A. & Bos J. I. B. Effector proteins that modulate plant-insect interactions. Curr Opin Plant Biol 14, 422–428 (2011).21684190 [PubMed]

Erb M., Meldau S. & Howe G. A. Role of phytohormones in insect-specific plant reactions. Trends Plant Sci 17, 250–259 (2012).22305233 [OpenAIRE] [PubMed]

Schaefer M.. Lipase activity in insect oral secretions mediates defense responses in Arabidopsis. Plant Physiol 156, 1520–1534 (2011).21546453 [OpenAIRE] [PubMed]

Sharma A.. Salivary proteins of plant-feeding hemipteroids-implication in phytophagy. B Entomol Res 104, 117–136 (2014).

Atamian H. S.. In planta expression or delivery of potato aphid Macrosiphum euphorbiae effectors Me10 and Me23 enhances aphid fecundity. Mol Plant Microbe Interact 26, 67–74 (2013).23194342 [PubMed]

Will T., Tjallingii W. F., Thonnessen A. & van Bel A. J. Molecular sabotage of plant defense by aphid saliva. Proc Natl Acad Sci USA 104, 10536–10541 (2007).17553961 [OpenAIRE] [PubMed]

Hattori M.. Molecular cloning of a novel calcium-binding protein in the secreted saliva of the green rice leafhopper Nephotettix cincticeps. Insect Biochem Mol Biol 42, 1–9 (2012).22019571 [PubMed]

Consales F.. Insect oral secretions suppress wound-induced responses in Arabidopsis. J EXP Bot 63, 727–737 (2012).21994175 [OpenAIRE] [PubMed]

Steinhorst L. & Kudla J. Signaling in cells and organisms-calcium holds the line. Curr Opin Plant Biol 22, 14–21 (2014).25195171 [PubMed]

Kudla J., Batistic O. & Hashimoto K. Calcium signals: the lead currency of plant information processing. Plant Cell 22, 541–563 (2010).20354197 [OpenAIRE] [PubMed]

Grabarek Z.Insights into modulation of calcium signaling by magnesium in calmodulin, troponin C and related EF-hand proteins. Biochim Biophys Acta 1813, 913–921 (2011).21262274 [OpenAIRE] [PubMed]

Poovaiah B. W., Du L., Wang H. & Yang T. Recent advances in calcium/calmodulin-mediated signaling with an em phasis on plant-microbe interactions. Plant Physiol 163, 531–542 (2013).24014576 [OpenAIRE] [PubMed]

Batistic O. & Kudla J. Analysis of calcium signaling pathways in plants. Biochim Biophys Acta 1820, 1283–1293 (2012).22061997 [PubMed]

50 references, page 1 of 4
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publication . Article . 2017

A salivary EF-hand calcium-binding protein of the brown planthopper Nilaparvata lugens functions as an effector for defense responses in rice

Ye, Wenfeng; Yu, Haixin; Jian, Yukun; Zeng, Jiamei; Ji, Rui; Chen, Hongdan; Lou, Yonggen;