publication . Article . 2012

Novel modulatory effects of neurosteroids and benzodiazepines on excitatory and inhibitory neurons excitability: a multi-electrode array recording study

Puia, Giulia; Gullo, Francesca; Dossi, Elena; Lecchi, Marzia; Wanke, Enzo;
Open Access English
  • Published: 01 Nov 2012 Journal: Frontiers in Neural Circuits, volume 6 (issn: 1662-5110, eissn: 1662-5110, Copyright policy)
  • Publisher: Frontiers Media S.A.
Abstract
The balance between glutamate- and GABA-mediated neurotransmission in the brain is fundamental in the nervous system, but it is regulated by the “tonic” release of a variety of endogenous factors. One such important group of molecules are the neurosteroids (NSs) which, similarly to benzodiazepines (BDZs), enhance GABAergic neurotransmission. The purpose of our work was to investigate, at in vivo physiologically relevant concentrations, the effects of NSs and BDZs as GABA modulators on dissociated neocortical neuron networks grown in long-term culture. We used a multi-electrode array (MEA) recording technique and a novel analysis that was able to both identify th...
Subjects
free text keywords: GABAA receptor modulators, benzodiazepines, neurosteroids, Neuroscience, neocortical cultures, Original Research Article, multi-electrode array
64 references, page 1 of 5

Agis-Balboa R. C. Pinna G. Zhubi A. Maloku E. Veldic M. Costa E. (2006). Characterization of brain neurons that express enzymes mediating neurosteroid biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 103 14602–14607. [OpenAIRE]

Arnold F. J. Hofmann F. Bengston C. P. Wittman M. Vanhoutte P. Bading H. (2005). Microelectrode arrays recordings of cultured hippocampal networks reveal a simple model for transcription and protein synthesis-dependent plasticity. J. Physiol. 56 4 3–19.

Akk G. Covey D. F. Evers A. S. Steinbach J. H. Zorumski C. F. Mennerick S. (2009). The influence of the membrane on neurosteroid action at GABA A receptors. Psychoneuroendocrinology 34(Suppl. 1) S59–S66. [OpenAIRE]

Bacci A. Huguenard J. R. (2006). Enhancement of spike-timing precision by autaptic transmission in neocortical inhibitory interneurons. Neuron 49 119–130. [OpenAIRE]

Baddeley R. Abbott L. F. Booth M. C. Sengpiel F. Freeman T. Wakeman E. A. (1997). Responses of neurons in primary and inferior temporal visual cortices to natural scenes. Proc. R. Soc. Lond. B Biol. Sci. 264 1775–1783. [OpenAIRE]

Baltz T. de Lima A. D. Voigt T. (2010). Contribution of GABAergic interneurons to the development of spontaneous activity patterns in cultured neocortical networks. Front. Cell. Neurosci. 4:15. 10.3389/fncel.2010.00015 [DOI]

Bartho P. Hirase H. Monconduit L. Zugaro M. Harris K. D. Buzsaki G. (2004). Characterization of neocortical principal cells and interneurons by network interactions and extracellular features. J. Neurophysiol. 92 600–608.

Becchetti A. Gullo F. Bruno G. Dossi E. Lecchi M. Wanke E. (2012). Exact distinction of excitatory and inhibitory neurons in neural networks: a study with GFP-GAD67 neurons optically and electrophysiologically recognized on multielectrode arrays. Front. Neural Circuits 6:63. 10.3389/fncir.2012.00063 [DOI]

Belelli D. Lambert J. J. (2005). Neurosteroids: endogenous regulators of the GABAA receptor. Nat. Rev. Neurosci. 6 565–575.

Berretta N. Bernardi G. Mercuri N. B. (2010). Firing properties and functional connectivity of substantia nigra pars compacta neurones recorded with a multi-electrodearray in vitro. J. Physiol. 588 1719–1735. [OpenAIRE]

Constantinidis C. Goldman-Rakic P. S. (2002). Correlated discharges among putative pyramidal neurons and interneurons in the primate prefrontal cortex. J. Neurophysiol. 88 3487–3497.

Cooper E. J. Johnston G. A. Edwards F. A. (1999). Effects of a naturally occurring neurosteroid on GABA A IPSCs during development in rat hippocampal or cerebellar slices. J. Physiol. (Lond.) 521 437–449.

Cope D. W. Hughes S. W. Crunelli V. (2005). GABA A receptor-mediated tonic inhibition in thalamic neurons. J. Neurosci. 25 11553–11563. [OpenAIRE]

Dhir A. Rogawski M. A. (2011). Role of neurosteroids in the anticonvulsant activity of midazolam. Br. J. Pharmacol. 165 2684–2691.

Dossi E. Heine C. Servettini I. Gullo F. Sygnecka K. Franke H. (2012). Functional regeneration of the ex-vivo reconstructed mesocorticolimbic dopaminergic system. Cereb. Cortex. 10.1093/cercor/bhs275 [Epub ahead of print]. [OpenAIRE] [DOI]

64 references, page 1 of 5
Similar Outcomes
20 research outcomes, page 1 of 2
20 research outcomes, page 1 of 2
Abstract
The balance between glutamate- and GABA-mediated neurotransmission in the brain is fundamental in the nervous system, but it is regulated by the “tonic” release of a variety of endogenous factors. One such important group of molecules are the neurosteroids (NSs) which, similarly to benzodiazepines (BDZs), enhance GABAergic neurotransmission. The purpose of our work was to investigate, at in vivo physiologically relevant concentrations, the effects of NSs and BDZs as GABA modulators on dissociated neocortical neuron networks grown in long-term culture. We used a multi-electrode array (MEA) recording technique and a novel analysis that was able to both identify th...
Subjects
free text keywords: GABAA receptor modulators, benzodiazepines, neurosteroids, Neuroscience, neocortical cultures, Original Research Article, multi-electrode array
64 references, page 1 of 5

Agis-Balboa R. C. Pinna G. Zhubi A. Maloku E. Veldic M. Costa E. (2006). Characterization of brain neurons that express enzymes mediating neurosteroid biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 103 14602–14607. [OpenAIRE]

Arnold F. J. Hofmann F. Bengston C. P. Wittman M. Vanhoutte P. Bading H. (2005). Microelectrode arrays recordings of cultured hippocampal networks reveal a simple model for transcription and protein synthesis-dependent plasticity. J. Physiol. 56 4 3–19.

Akk G. Covey D. F. Evers A. S. Steinbach J. H. Zorumski C. F. Mennerick S. (2009). The influence of the membrane on neurosteroid action at GABA A receptors. Psychoneuroendocrinology 34(Suppl. 1) S59–S66. [OpenAIRE]

Bacci A. Huguenard J. R. (2006). Enhancement of spike-timing precision by autaptic transmission in neocortical inhibitory interneurons. Neuron 49 119–130. [OpenAIRE]

Baddeley R. Abbott L. F. Booth M. C. Sengpiel F. Freeman T. Wakeman E. A. (1997). Responses of neurons in primary and inferior temporal visual cortices to natural scenes. Proc. R. Soc. Lond. B Biol. Sci. 264 1775–1783. [OpenAIRE]

Baltz T. de Lima A. D. Voigt T. (2010). Contribution of GABAergic interneurons to the development of spontaneous activity patterns in cultured neocortical networks. Front. Cell. Neurosci. 4:15. 10.3389/fncel.2010.00015 [DOI]

Bartho P. Hirase H. Monconduit L. Zugaro M. Harris K. D. Buzsaki G. (2004). Characterization of neocortical principal cells and interneurons by network interactions and extracellular features. J. Neurophysiol. 92 600–608.

Becchetti A. Gullo F. Bruno G. Dossi E. Lecchi M. Wanke E. (2012). Exact distinction of excitatory and inhibitory neurons in neural networks: a study with GFP-GAD67 neurons optically and electrophysiologically recognized on multielectrode arrays. Front. Neural Circuits 6:63. 10.3389/fncir.2012.00063 [DOI]

Belelli D. Lambert J. J. (2005). Neurosteroids: endogenous regulators of the GABAA receptor. Nat. Rev. Neurosci. 6 565–575.

Berretta N. Bernardi G. Mercuri N. B. (2010). Firing properties and functional connectivity of substantia nigra pars compacta neurones recorded with a multi-electrodearray in vitro. J. Physiol. 588 1719–1735. [OpenAIRE]

Constantinidis C. Goldman-Rakic P. S. (2002). Correlated discharges among putative pyramidal neurons and interneurons in the primate prefrontal cortex. J. Neurophysiol. 88 3487–3497.

Cooper E. J. Johnston G. A. Edwards F. A. (1999). Effects of a naturally occurring neurosteroid on GABA A IPSCs during development in rat hippocampal or cerebellar slices. J. Physiol. (Lond.) 521 437–449.

Cope D. W. Hughes S. W. Crunelli V. (2005). GABA A receptor-mediated tonic inhibition in thalamic neurons. J. Neurosci. 25 11553–11563. [OpenAIRE]

Dhir A. Rogawski M. A. (2011). Role of neurosteroids in the anticonvulsant activity of midazolam. Br. J. Pharmacol. 165 2684–2691.

Dossi E. Heine C. Servettini I. Gullo F. Sygnecka K. Franke H. (2012). Functional regeneration of the ex-vivo reconstructed mesocorticolimbic dopaminergic system. Cereb. Cortex. 10.1093/cercor/bhs275 [Epub ahead of print]. [OpenAIRE] [DOI]

64 references, page 1 of 5
Similar Outcomes
20 research outcomes, page 1 of 2
20 research outcomes, page 1 of 2
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publication . Article . 2012

Novel modulatory effects of neurosteroids and benzodiazepines on excitatory and inhibitory neurons excitability: a multi-electrode array recording study

Puia, Giulia; Gullo, Francesca; Dossi, Elena; Lecchi, Marzia; Wanke, Enzo;