publication . Article . 2017

The microbiota of water buffalo milk during mastitis

Catozzi, Carlotta; Sanchez Bonastre, Armand; Francino, Olga; Lecchi, Cristina; De Carlo, Esterina; Vecchio, Domenico; Martucciello, Alessandra; Fraulo, Pasquale; Bronzo, Valerio; Cuscó, Anna; ...
Open Access English
  • Published: 19 Sep 2017 Journal: PLoS ONE, volume 12, issue 9 (issn: 1932-6203, eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science
Abstract
The aim of this study was to define the microbiota of water buffalo milk during sub-clinical and clinical mastitis, as compared to healthy status, by using high-throughput sequencing of the 16S rRNA gene. A total of 137 quarter samples were included in the experimental design: 27 samples derived from healthy, culture negative quarters, with a Somatic Cell Count (SCC) of less than 200,000 cells/ml; 27 samples from quarters with clinical mastitis; 83 samples were collected from quarters with subclinical mastitis, with a SCC number greater of 200,000 cells/ml and/or culture positive for udder pathogens, without clinical signs of mastitis. Bacterial DNA was purified...
Subjects
free text keywords: Microbial Pathogens, Research Article, Bacteria, Anatomy, Pathology and Laboratory Medicine, Fusobacteria, Gut Bacteria, Ribosomes, Genetics, Pathogens, Staphylococcus, Genomics, Diet, Settore VET/05 - Malattie Infettive degli Animali Domestici, Beverages, Biology and Life Sciences, Non-coding RNA, Mastitis, RNA, Milk, Physiology, Microbiology, Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria, Medicine, Maternal Health, Body Fluids, Nucleic acids, Bacterial Pathogens, Microbiome, Microbial Genomics, Q, Medical Microbiology, R, Nutrition, Cell biology, Cellular structures and organelles, Bovine Mastitis, Science, Women's Health, Biochemistry, Ribosomal RNA, Organisms, Medicine and Health Sciences, Animal Diseases, Zoology
64 references, page 1 of 5

1 Stubbendieck RM, Vargas-Bautista C, Straight PD. Bacterial Communities: Interactions to Scale. Front Microbiol. 2016;7: 1234 doi: 10.3389/fmicb.2016.01234 27551280 [OpenAIRE] [PubMed]

2 Thaiss CA, Zmora N, Levy M, Elinav E. The microbiome and innate immunity. Nature. 2016;535: 65–74. doi: 10.1038/nature18847 27383981 [PubMed]

3 Rogers GB, Hoffman LR, Carroll MP, Bruce KD. Interpreting infective microbiota: the importance of an ecological perspective. Trends Microbiol. 2013;21: 271–276. doi: 10.1016/j.tim.2013.03.004 23598051 [OpenAIRE] [PubMed]

4 Vayssier-Taussat M, Albina E, Citti C, Cosson JF, Jacques MA, Lebrun MH, et al Shifting the paradigm from pathogens to pathobiome: new concepts in the light of meta-omics. Front Cell Infect Microbiol. 2014;4: 29 doi: 10.3389/fcimb.2014.00029 24634890 [OpenAIRE] [PubMed]

5 Weimer PJ. Redundancy, resilience, and host specificity of the ruminal microbiota: implications for engineering improved ruminal fermentations. Front Microbiol. 2015;6: 296 doi: 10.3389/fmicb.2015.00296 25914693 [OpenAIRE] [PubMed]

6 Jami E, White BA, Mizrahi I. Potential role of the bovine rumen microbiome in modulating milk composition and feed efficiency. PLoS One. 2014;9: e85423 doi: 10.1371/journal.pone.0085423 24465556 [OpenAIRE] [PubMed]

7 Henderson G, Cox F, Ganesh S, Jonker A, Young W; Global Rumen Census Collaborators, Janssen PH. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep. 2015;5: 14567 doi: 10.1038/srep14567 26449758 [OpenAIRE] [PubMed]

8 Kumar S, Indugu N, Vecchiarelli B, Pitta DW. Associative patterns among anaerobic fungi, methanogenic archaea, and bacterial communities in response to changes in diet and age in the rumen of dairy cows. Front Microbiol. 2015;6: 781 doi: 10.3389/fmicb.2015.00781 26284058 [OpenAIRE] [PubMed]

9 Myer PR, Smith TP, Wells JE, Kuehn LA, Freetly HC. Rumen microbiome from steers differing in feed efficiency. PLoS One. 2015;10: e0129174 doi: 10.1371/journal.pone.0129174 26030887 [OpenAIRE] [PubMed]

10 Santos TM, Gilbert RO, Bicalho RC. Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows. J Dairy Sci. 2011;94: 291–302. doi: 10.3168/jds.2010-3668 21183039 [PubMed]

11 Santos TM, Bicalho RC. Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows. PLoS One. 2012;7: e53048 doi: 10.1371/journal.pone.0053048 23300859 [OpenAIRE] [PubMed]

12 Machado VS, Oikonomou G, Bicalho ML, Knauer WA, Gilbert R, Bicalho RC. Investigation of postpartum dairy cows' uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene. Vet Microbiol. 2012;159: 460–469. doi: 10.1016/j.vetmic.2012.04.033 22595139 [PubMed]

13 Jeon SJ, Vieira-Neto A, Gobikrushanth M, Daetz R, Mingoti RD, Parize AC, de Freitas SL, et al Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows. Appl Environ Microbiol. 2015;81: 6324–6332. doi: 10.1128/AEM.01753-15 26150453 [OpenAIRE] [PubMed]

14 Rodrigues NF, Kästle J, Coutinho TJ, Amorim AT, Campos GB, Santos VM, et al Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease. Genet Mol Res. 2015;14: 6518–6528. doi: 10.4238/2015.June.12.4 26125856 [OpenAIRE] [PubMed]

15 Oikonomou G, Machado VS, Santisteban C, Schukken YH, Bicalho RC. Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomics 16s rDNA. PLoS One. 2012;7: e47671 doi: 10.1371/journal.pone.0047671 23082192 [OpenAIRE] [PubMed]

64 references, page 1 of 5
Abstract
The aim of this study was to define the microbiota of water buffalo milk during sub-clinical and clinical mastitis, as compared to healthy status, by using high-throughput sequencing of the 16S rRNA gene. A total of 137 quarter samples were included in the experimental design: 27 samples derived from healthy, culture negative quarters, with a Somatic Cell Count (SCC) of less than 200,000 cells/ml; 27 samples from quarters with clinical mastitis; 83 samples were collected from quarters with subclinical mastitis, with a SCC number greater of 200,000 cells/ml and/or culture positive for udder pathogens, without clinical signs of mastitis. Bacterial DNA was purified...
Subjects
free text keywords: Microbial Pathogens, Research Article, Bacteria, Anatomy, Pathology and Laboratory Medicine, Fusobacteria, Gut Bacteria, Ribosomes, Genetics, Pathogens, Staphylococcus, Genomics, Diet, Settore VET/05 - Malattie Infettive degli Animali Domestici, Beverages, Biology and Life Sciences, Non-coding RNA, Mastitis, RNA, Milk, Physiology, Microbiology, Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria, Medicine, Maternal Health, Body Fluids, Nucleic acids, Bacterial Pathogens, Microbiome, Microbial Genomics, Q, Medical Microbiology, R, Nutrition, Cell biology, Cellular structures and organelles, Bovine Mastitis, Science, Women's Health, Biochemistry, Ribosomal RNA, Organisms, Medicine and Health Sciences, Animal Diseases, Zoology
64 references, page 1 of 5

1 Stubbendieck RM, Vargas-Bautista C, Straight PD. Bacterial Communities: Interactions to Scale. Front Microbiol. 2016;7: 1234 doi: 10.3389/fmicb.2016.01234 27551280 [OpenAIRE] [PubMed]

2 Thaiss CA, Zmora N, Levy M, Elinav E. The microbiome and innate immunity. Nature. 2016;535: 65–74. doi: 10.1038/nature18847 27383981 [PubMed]

3 Rogers GB, Hoffman LR, Carroll MP, Bruce KD. Interpreting infective microbiota: the importance of an ecological perspective. Trends Microbiol. 2013;21: 271–276. doi: 10.1016/j.tim.2013.03.004 23598051 [OpenAIRE] [PubMed]

4 Vayssier-Taussat M, Albina E, Citti C, Cosson JF, Jacques MA, Lebrun MH, et al Shifting the paradigm from pathogens to pathobiome: new concepts in the light of meta-omics. Front Cell Infect Microbiol. 2014;4: 29 doi: 10.3389/fcimb.2014.00029 24634890 [OpenAIRE] [PubMed]

5 Weimer PJ. Redundancy, resilience, and host specificity of the ruminal microbiota: implications for engineering improved ruminal fermentations. Front Microbiol. 2015;6: 296 doi: 10.3389/fmicb.2015.00296 25914693 [OpenAIRE] [PubMed]

6 Jami E, White BA, Mizrahi I. Potential role of the bovine rumen microbiome in modulating milk composition and feed efficiency. PLoS One. 2014;9: e85423 doi: 10.1371/journal.pone.0085423 24465556 [OpenAIRE] [PubMed]

7 Henderson G, Cox F, Ganesh S, Jonker A, Young W; Global Rumen Census Collaborators, Janssen PH. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep. 2015;5: 14567 doi: 10.1038/srep14567 26449758 [OpenAIRE] [PubMed]

8 Kumar S, Indugu N, Vecchiarelli B, Pitta DW. Associative patterns among anaerobic fungi, methanogenic archaea, and bacterial communities in response to changes in diet and age in the rumen of dairy cows. Front Microbiol. 2015;6: 781 doi: 10.3389/fmicb.2015.00781 26284058 [OpenAIRE] [PubMed]

9 Myer PR, Smith TP, Wells JE, Kuehn LA, Freetly HC. Rumen microbiome from steers differing in feed efficiency. PLoS One. 2015;10: e0129174 doi: 10.1371/journal.pone.0129174 26030887 [OpenAIRE] [PubMed]

10 Santos TM, Gilbert RO, Bicalho RC. Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows. J Dairy Sci. 2011;94: 291–302. doi: 10.3168/jds.2010-3668 21183039 [PubMed]

11 Santos TM, Bicalho RC. Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows. PLoS One. 2012;7: e53048 doi: 10.1371/journal.pone.0053048 23300859 [OpenAIRE] [PubMed]

12 Machado VS, Oikonomou G, Bicalho ML, Knauer WA, Gilbert R, Bicalho RC. Investigation of postpartum dairy cows' uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene. Vet Microbiol. 2012;159: 460–469. doi: 10.1016/j.vetmic.2012.04.033 22595139 [PubMed]

13 Jeon SJ, Vieira-Neto A, Gobikrushanth M, Daetz R, Mingoti RD, Parize AC, de Freitas SL, et al Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows. Appl Environ Microbiol. 2015;81: 6324–6332. doi: 10.1128/AEM.01753-15 26150453 [OpenAIRE] [PubMed]

14 Rodrigues NF, Kästle J, Coutinho TJ, Amorim AT, Campos GB, Santos VM, et al Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease. Genet Mol Res. 2015;14: 6518–6528. doi: 10.4238/2015.June.12.4 26125856 [OpenAIRE] [PubMed]

15 Oikonomou G, Machado VS, Santisteban C, Schukken YH, Bicalho RC. Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomics 16s rDNA. PLoS One. 2012;7: e47671 doi: 10.1371/journal.pone.0047671 23082192 [OpenAIRE] [PubMed]

64 references, page 1 of 5
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publication . Article . 2017

The microbiota of water buffalo milk during mastitis

Catozzi, Carlotta; Sanchez Bonastre, Armand; Francino, Olga; Lecchi, Cristina; De Carlo, Esterina; Vecchio, Domenico; Martucciello, Alessandra; Fraulo, Pasquale; Bronzo, Valerio; Cuscó, Anna; ...