A Method to Identify and Isolate Pluripotent Human Stem Cells and Mouse Epiblast Stem Cells Using Lipid Body-Associated Retinyl Ester Fluorescence

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Thangaselvam Muthusamy ; Odity Mukherjee ; Radhika Menon ; Megha Prakash Bangalore ; Mitradas M. Panicker (2014)

Summary We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450–500 nm) is readily observed by fluorescence microscopy and corr... View more
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