A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules.

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Czimmerer, Zsolt; Hulvely, Julianna; Simandi, Zoltan; Varallyay, Eva; Havelda, Zoltan; Szabo, Erzsebet; Varga, Attila; Dezso, Balazs; Balogh, Maria; Horvath, Attila; Domokos, Balint; Torok, Zsolt; Nagy, Laszlo; Balint, Balint L.;
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE,volume 8,issue 1 (issn: 1932-6203, eissn: 1932-6203)
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  • Related identifiers: pmc: PMC3561390, doi: 10.1371/journal.pone.0055168
  • Subject: Elméleti orvostudományok | Orvostudományok | Research Article | Biology | Molecular cell biology | RNA | Medicine | Nucleic acids | RNA processing | Epigenetics | Q | R | Genetics | RNA stability | Biochemistry | Science | RNA interference | Gene expression

Short regulatory RNA-s have been identified as key regulators of gene expression in eukaryotes. They have been involved in the regulation of both physiological and pathological processes such as embryonal development, immunoregulation and cancer. One of their relevant c... View more
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