Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments

Preprint, Other literature type English OPEN
Shalem, Ophir; Zhang, Feng; Pinello, Luca; Concordet, Jean-Paul; Canver, Matthew; Haeussler, Maximilian; Bauer, Daniel; Orkin, Stuart; Sanjana, Neville; Yuan, Guo-Cheng;

CRISPR (clustered regularly interspaced short palindromic repeats) genome-editing experiments offer enormous potential for the evaluation of genomic loci using arrayed single guide RNARNARNAs (sgRNAs) or pooled sgRNA libraries. Numerous computational tools are available... View more