Structure-Guided Design of an Engineered Streptavidin with Reusability to Purify Streptavidin-Binding Peptide Tagged Proteins or Biotinylated Proteins

Article English OPEN
Sau-Ching Wu; Sui-Lam Wong;
(2013)
  • Publisher: Public Library of Science
  • Journal: PLoS ONE, volume 8, issue 7 (issn: 1932-6203, eissn: 1932-6203)
  • Publisher copyright policies & self-archiving
  • Identifiers: pmc: PMC3712923, doi: 10.1371/journal.pone.0069530
  • Subject: Chemistry | Affinity Chromatography | Biotechnology | Research Article | Biology | Proteomics | Size Exclusion Chromatography | Recombinant Proteins | Microbiology | Medicine | Biomacromolecule-Ligand Interactions | Applied Microbiology | Protein Interactions | Protein Structure | Q | Synthetic Biology | R | Protein Engineering | Proteins | Science | Biochemistry | Chromatography | Synthetic Peptide

Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged recombinant proteins to be affinity purified using the streptavidin matrix without the need of biotinylation. The major limitation of this powerful technology is the requirement to u... View more
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