Protoplast isolation and culture from Kalanchoë species:optimization of plant growth regulator concentration for efficient callus production

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Cui, Jin; Mackenzie, Kathryn; Eeckhaut, Tom; Müller, Renate; Lütken, Henrik;
  • Journal: volume 138, pages 287-297issn: 0167-6857
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  • Identifiers: doi: 10.1007/s11240-019-01624-4
  • Subject: Protoplast isolation | Plant growth regulators | Protoplast-derived callus | REGENERATION | SHOOT ELONGATION | Plant regeneration | PROGRESS | INDUCED INHIBITION | HYBRIDS | LEAF | Organogenesis | 1182 Biochemistry, cell and molecular biology

A high yield of isolated protoplasts and efficient regeneration protocols are prerequisites for successful development of somatic hybrids. In the present study, protoplast isolation and regeneration were evaluated in 12 Kalanchoe accessions belonging to nine species. Th... View more
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