publication . Article . 2017

RecG controls DNA amplification at double‐strand breaks and arrested replication forks

Azeroglu, Benura; Leach, David R F;
Open Access English
  • Published: 01 Feb 2017 Journal: Febs Letters, volume 591, issue 8, pages 1,101-1,113 (issn: 0014-5793, eissn: 1873-3468, Copyright policy)
  • Publisher: John Wiley and Sons Inc.
Abstract
<p>DNA amplification is a powerful mutational mechanism that is a hallmark of cancer and drug resistance. It is therefore important to understand the fundamental pathways that cells employ to avoid over-replicating sections of their genomes. Recent studies demonstrate that, in the absence of RecG, DNA amplification is observed at sites of DNA double-strand break repair (DSBR) and of DNA replication arrest that are processed to generate double-strand ends. RecG also plays a role in stabilising joint molecules formed during DSBR. We propose that RecG prevents a previously unrecognised mechanism of DNA amplification that we call reverse-restart, which generates DNA...
Subjects
free text keywords: Review Articles, Focus on… DNA Recombination | Genome organization and stability, DNA amplification, Review Article, RecG, Review, double‐strand break repair, Journal Article
113 references, page 1 of 8

1 Lloyd RG (1991) Conjugational recombination in resolvase‐deficient ruvC mutants of Escherichia coli K‐12 depends on recG . J Bacteriol 173, 5414–5418.1653210 [OpenAIRE] [PubMed]

2 Lloyd RG and Sharples GJ (1993) Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli . Nucleic Acids Res 21, 1719–1725.8388095 [OpenAIRE] [PubMed]

3 Lloyd RG and Sharples GJ (1993) Dissociation of synthetic Holliday junctions by E. coli RecG protein. EMBO J 12, 17–22.8428576 [OpenAIRE] [PubMed]

4 Mandal TN, Mahdi AA, Sharples GJ and Lloyd RG (1993) Resolution of Holliday intermediates in recombination and DNA repair: indirect suppression of ruvA, ruvB, and ruvC mutations. J Bacteriol 175, 4325–4334.8331065 [OpenAIRE] [PubMed]

5 Meddows TR, Savory AP and Lloyd RG (2004) RecG helicase promotes DNA double‐strand break repair. Mol Microbiol 52, 119–132.15049815 [PubMed]

6 Wardr ope L, Okely E and Leach D (2009) Resolution of joint molecules by RuvABC and RecG following cleavage of the Escherichia coli chromosome by EcoKI. PLoS One 4, e6542.19657385 [OpenAIRE] [PubMed]

7 Whitby MC and Lloyd RG (1995) Branch migration of three‐strand recombination intermediates by RecG, a possible pathway for securing exchanges initiated by 3′‐tailed duplex DNA. EMBO J 14, 3302–3310.7628432 [OpenAIRE] [PubMed]

8 Whitby MC, Ryder L and Lloyd RG (1993) Reverse branch migration of Holliday junctions by RecG protein: a new mechanism for resolution of intermediates in recombination and DNA repair. Cell 75, 341–350.8402917 [PubMed]

9 Abd Wahab S, Choi M and Bianco PR (2013) Characterization of the ATPase activity of RecG and RuvAB proteins on model fork structures reveals insight into stalled DNA replication fork repair. J Biol Chem 288, 26397–26409.23893472 [OpenAIRE] [PubMed]

10 Buss JA, Kimura Y and Bianco PR (2008) RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic Acids Res 36, 7029–7042.18986999 [OpenAIRE] [PubMed]

11 Gupta S, Yeeles JT and Marians KJ (2014) Regression of replication forks stalled by leading‐strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially. J Biol Ch em 289, 28376–28387.25138216 [OpenAIRE] [PubMed]

12 Manosas M, Perumal SK, Bianco P, Ritort F, Benkovic SJ and Croquette V (2013) RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue. Nat Commun 4, 2368.24013402 [OpenAIRE] [PubMed]

13 McGlynn P and Lloyd RG (1999) RecG helicase activity at three‐ and four‐strand DNA structures. Nucleic Acids Res 27, 3049–3056.10454599 [OpenAIRE] [PubMed]

14 McGlynn P and Lloyd RG (2000) Modulati on of RNA polymerase by (p)ppGpp reveals a RecG‐dependent mechanism for replication fork progression. Cell 101, 35–45.10778854 [PubMed]

15 McGlynn P and Lloyd RG (2001) Rescue of stalled replication forks by RecG: simultaneous translocation on the leading and lagging strand templates supports an active DNA unwinding model of fork reversal and Holliday junction formation. Proc Natl Acad Sci USA 98, 8227–8234.11459957 [OpenAIRE] [PubMed]

113 references, page 1 of 8
Abstract
<p>DNA amplification is a powerful mutational mechanism that is a hallmark of cancer and drug resistance. It is therefore important to understand the fundamental pathways that cells employ to avoid over-replicating sections of their genomes. Recent studies demonstrate that, in the absence of RecG, DNA amplification is observed at sites of DNA double-strand break repair (DSBR) and of DNA replication arrest that are processed to generate double-strand ends. RecG also plays a role in stabilising joint molecules formed during DSBR. We propose that RecG prevents a previously unrecognised mechanism of DNA amplification that we call reverse-restart, which generates DNA...
Subjects
free text keywords: Review Articles, Focus on… DNA Recombination | Genome organization and stability, DNA amplification, Review Article, RecG, Review, double‐strand break repair, Journal Article
113 references, page 1 of 8

1 Lloyd RG (1991) Conjugational recombination in resolvase‐deficient ruvC mutants of Escherichia coli K‐12 depends on recG . J Bacteriol 173, 5414–5418.1653210 [OpenAIRE] [PubMed]

2 Lloyd RG and Sharples GJ (1993) Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli . Nucleic Acids Res 21, 1719–1725.8388095 [OpenAIRE] [PubMed]

3 Lloyd RG and Sharples GJ (1993) Dissociation of synthetic Holliday junctions by E. coli RecG protein. EMBO J 12, 17–22.8428576 [OpenAIRE] [PubMed]

4 Mandal TN, Mahdi AA, Sharples GJ and Lloyd RG (1993) Resolution of Holliday intermediates in recombination and DNA repair: indirect suppression of ruvA, ruvB, and ruvC mutations. J Bacteriol 175, 4325–4334.8331065 [OpenAIRE] [PubMed]

5 Meddows TR, Savory AP and Lloyd RG (2004) RecG helicase promotes DNA double‐strand break repair. Mol Microbiol 52, 119–132.15049815 [PubMed]

6 Wardr ope L, Okely E and Leach D (2009) Resolution of joint molecules by RuvABC and RecG following cleavage of the Escherichia coli chromosome by EcoKI. PLoS One 4, e6542.19657385 [OpenAIRE] [PubMed]

7 Whitby MC and Lloyd RG (1995) Branch migration of three‐strand recombination intermediates by RecG, a possible pathway for securing exchanges initiated by 3′‐tailed duplex DNA. EMBO J 14, 3302–3310.7628432 [OpenAIRE] [PubMed]

8 Whitby MC, Ryder L and Lloyd RG (1993) Reverse branch migration of Holliday junctions by RecG protein: a new mechanism for resolution of intermediates in recombination and DNA repair. Cell 75, 341–350.8402917 [PubMed]

9 Abd Wahab S, Choi M and Bianco PR (2013) Characterization of the ATPase activity of RecG and RuvAB proteins on model fork structures reveals insight into stalled DNA replication fork repair. J Biol Chem 288, 26397–26409.23893472 [OpenAIRE] [PubMed]

10 Buss JA, Kimura Y and Bianco PR (2008) RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic Acids Res 36, 7029–7042.18986999 [OpenAIRE] [PubMed]

11 Gupta S, Yeeles JT and Marians KJ (2014) Regression of replication forks stalled by leading‐strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially. J Biol Ch em 289, 28376–28387.25138216 [OpenAIRE] [PubMed]

12 Manosas M, Perumal SK, Bianco P, Ritort F, Benkovic SJ and Croquette V (2013) RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue. Nat Commun 4, 2368.24013402 [OpenAIRE] [PubMed]

13 McGlynn P and Lloyd RG (1999) RecG helicase activity at three‐ and four‐strand DNA structures. Nucleic Acids Res 27, 3049–3056.10454599 [OpenAIRE] [PubMed]

14 McGlynn P and Lloyd RG (2000) Modulati on of RNA polymerase by (p)ppGpp reveals a RecG‐dependent mechanism for replication fork progression. Cell 101, 35–45.10778854 [PubMed]

15 McGlynn P and Lloyd RG (2001) Rescue of stalled replication forks by RecG: simultaneous translocation on the leading and lagging strand templates supports an active DNA unwinding model of fork reversal and Holliday junction formation. Proc Natl Acad Sci USA 98, 8227–8234.11459957 [OpenAIRE] [PubMed]

113 references, page 1 of 8
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publication . Article . 2017

RecG controls DNA amplification at double‐strand breaks and arrested replication forks

Azeroglu, Benura; Leach, David R F;