Production of heterologous cutinases by E. coli and improved enzyme formulation for application on plastic degradation

Article English OPEN
Gomes,Daniela S; Matamá,Teresa; Cavaco-Paulo,Artur; Campos-Takaki,Galba M; Salgueiro,Alexandra A;

Background: The hydrolytic action of cutinases has been applied to the degradation of plastics. Polyethylene terephthalate (PET) have long half-life which constitutes a major problem for their treatment as urban solid residues. The aim of this work was to characterize a... View more
  • References (31)
    31 references, page 1 of 4

    AWAJA, F. and PAVEL, D. (2005). Recycling of PET. European Polymer Journal, vol. 41, no. 7, p. 1453-1477. [CrossRef]

    BARBOZA, E.S.; LÓPEZ, D.R.; AMICO, S.C. and FERREIRA, C.A. (2009). Determination of a recyclability index for the PET glycolysis. Resources, Conservation and Recycling, vol. 53, no. 3, p. 122-128. [CrossRef]

    CANEVAROLO, S.V. (2006). Ciencia dos polímeros: Um texto básico para tecnólogos engenheiros. Artliber. 280 p. ISBN 9788588098107.

    CHEN, S.; TONG, X.; WOODARD, R.W.; DU, G.; WU, J. and CHEN, J. (2008). Identification and characterization of bacterial cutinase. The Journal of Biological Chemistry, vol. 283, no. 38, p. 25854-25862. [CrossRef]

    EGMOND, M.R. and DE VLIEG, J. (2000). Fusarium solani pisi cutinase. Biochimie, vol. 82, no. 11, p. 1015-1021. [CrossRef]

    EINSFELDT, K.; JÚNIOR, J.B.S.; ARGONDIZZO, A.P.C.; MEDEIROS, M.A.; ALVES, T.L.M.; ALMEIDA, R.V. and LARENTIS, A.L. (2011). Cloning and expression of protease ClpP from Streptococcus pneumoniae in Escherichia coli: Study of the influence of kanamycin and IPTG concentration on cell growth, recombinant protein production and plasmid stability. Vaccine, vol. 29, no. 41, p. 7136-7143. [CrossRef]

    ESPINO-RAMMER, L.; RIBITSCH, D.; PRZYLUCKA, A.; MAROLD, A.; GREIMEL, K.J.; HERRERO-ACERO, E.; GUEBITZ, G.M.; KUBICEK, C.P. and DRUZHININA, I.S. (2013). Two novel class II hydrophobins from Trichoderma spp. stimulate enzymatic hydrolysis of poly(ethylene terephthalate) when expressed as fusion proteins. Applied and Environmental Microbiology, vol. 79, no. 14, p. 4230-4238. [CrossRef]

    HAMADA, H.; ARAKAWA, T. and SHIRAKI, K. (2009). Effect of additives on protein aggregation. Current Pharmaceutical Biotechnology, vol. 10, no. 4, p. 400-407. [CrossRef]

    HAN, M.J. and LEE, S.Y. (2006). The Escherichia coli proteome: Past, present, and future prospects. Microbiology and Molecular Biology Reviews, vol. 70, no. 2, p. 362-439. [CrossRef]

    HERRERO-ACERO, E.; RIBITSCH, D.; STEINKELLNER, G.; GRUBER, K.; GREIMEL, K.; EITELJOERG, I.; TROTSCHA, E.; WEI, R.; ZIMMERMANN, W.; ZINN, M.; CAVACO-PAULO, A.; FREDDI, G.; SCHWAB, H. and GUEBITZ, G. (2011). Enzymatic surface hydrolysis of PET: Effect of structural diversity on kinetic properties of cutinases from Thermobifida. Macromolecules, vol. 44, no. 12, p. 4632-4640. [CrossRef]

  • Related Organizations (1)
  • Metrics