publication . Article . Other literature type . 2012

Distinct Single-Cell Morphological Dynamics under Beta-Lactam Antibiotics

Roy Kishony; Zhizhong Yao; Daniel Kahne;
Open Access
  • Published: 01 Dec 2012 Journal: Molecular Cell, volume 48, pages 705-712 (issn: 1097-2765, Copyright policy)
  • Publisher: Elsevier BV
Abstract
The bacterial cell wall is conserved in prokaryotes, stabilizing cells against osmotic stress. Beta-lactams inhibit cell-wall synthesis and induce lysis through a bulge-mediated mechanism; however, little is known about the formation dynamics and stability of these bulges. To capture processes of different timescales, we developed an imaging platform combining automated image analysis with live-cell microscopy at high time resolution. Beta-lactam killing of Escherichia coli cells proceeded through four stages: elongation, bulge formation, bulge stagnation, and lysis. Both the cell wall and outer membrane (OM) affect the observed dynamics; damaging the cell wall ...
Subjects
free text keywords: Cell Biology, Molecular Biology, Article, Bacterial outer membrane, Microbiology, Elongation, Bulge, Bacterial cell structure, Osmotic shock, Cell wall, Lysis, Cell, medicine.anatomical_structure, medicine, Biophysics, Biology
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