publication . Article . 2004

A role for the actin cytoskeleton in cell death and aging in yeast

Gourlay, C.W.; Carpp, L.N.; Timpson, P.; Winder, S.J.; Ayscough, K.R.;
Open Access English
  • Published: 15 Mar 2004 Journal: The Journal of Cell Biology, volume 164, issue 6, pages 803-809 (issn: 0021-9525, eissn: 1540-8140, Copyright policy)
  • Publisher: The Rockefeller University Press
  • Country: United Kingdom
Abstract
<jats:p>Several determinants of aging, including metabolic capacity and genetic stability, are recognized in both yeast and humans. However, many aspects of the pathways leading to cell death remain to be elucidated. Here we report a role for the actin cytoskeleton both in cell death and in promoting longevity. We have analyzed yeast strains expressing mutants with either increased or decreased actin dynamics. We show that decreased actin dynamics causes depolarization of the mitochondrial membrane and an increase in reactive oxygen species (ROS) production, resulting in cell death. Important, however, is the demonstration that increasing actin dynamics, either ...
Subjects
Medical Subject Headings: macromolecular substances
free text keywords: Report, actin; senescence; Scp1; apoptosis; ROS, Q, Cell Biology
29 references, page 1 of 2

Ayscough, K.R. 2000. Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton. Curr. Biol. 10:1587-1590. [OpenAIRE]

Belmont, L.D., and D.G. Drubin. 1998. The yeast V159N actin mutant reveals roles for actin dynamics in vivo. J. Cell Biol. 142:1289-1299. [OpenAIRE]

Belmont, L.D., G.M. Patterson, and D.G. Drubin. 1999. New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites. J. Cell Sci. 112:1325-1336.

Boldogh, I.R., N. Vojtov, S. Karmon, and L.A. Pon. 1998. Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p. J. Cell Biol. 141:1371-1381. [OpenAIRE]

Frohlich, K.U., and F. Madeo. 2001. Apoptosis in yeast: a new model for aging research. Exp. Gerontol. 37:27-31.

Gonos, E.S., A. Derventzi, M. Kveiborg, G. Agiostratidou, M. Kassem, B.F.C. Clark, P.S. Jat, and S.I.S. Rattan. 1998. Cloning and identification of genes that associate with mammalian replicative senescence. Exp. Cell Res. 240:66-74.

Goodman, A., B.L. Goode, P. Matsudaira, and G.R. Fink. 2003. The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol. Biol. Cell. 14:2617-2629.

Guthrie, G., and G.R. Fink. 1991. Generation of rho yeast strains. Methods Enzymol. 194:150-151.

Hagan, I.M., and K.R. Ayscough. 2000. Fluorescence microscopy in yeast. In Protein Localization by Fluorescence Microscopy: A Practical Approach. V.J. Allan, editor. Oxford University Press, Oxford, UK. 179-205.

Hampsey, M. 1997. A review of phenotypes in Saccharomyces cerevisiae. Yeast. 13: 1099-1133. [OpenAIRE]

Jazwinski, S.M. 2002. Growing old: metabolic control and yeast aging. Annu. Rev. Microbiol. 56:769-792. [OpenAIRE]

Kim, S., S.G. Hwang, I.C. Kim, and J.S. Chun. 2003. Actin cytoskeletal architecture regulates nitric oxide-induced apoptosis, dedifferentiation and cyclooxygenase-2 expression in articular chondrocytes via mitogen-activated protein kinase and protein kinase C pathways. J. Biol. Chem. 278:42448-42456.

Kusano, H., S. Shimizu, R.C. Koya, H. Fujita, S. Kamada, N. Kuzumaki, and Y. Tsujimoto. 2000. Human gelsolin prevents apoptosis by inhibiting apoptotic mitochondrial changes via closing VDAC. Oncogene. 19:4807-4814. [OpenAIRE]

Laun, P., A. Pichova, F. Madeo, J. Fuchs, A. Ellinger, S. Kohlwein, I. Dawes, and K. Froehlich. 2001. Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis. Mol. Microbiol. 39:1166-1173. [OpenAIRE]

Lin, S.J., P.A. Defossez, and L. Guarente. 2000. Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science. 289:2126-2128.

29 references, page 1 of 2
Abstract
<jats:p>Several determinants of aging, including metabolic capacity and genetic stability, are recognized in both yeast and humans. However, many aspects of the pathways leading to cell death remain to be elucidated. Here we report a role for the actin cytoskeleton both in cell death and in promoting longevity. We have analyzed yeast strains expressing mutants with either increased or decreased actin dynamics. We show that decreased actin dynamics causes depolarization of the mitochondrial membrane and an increase in reactive oxygen species (ROS) production, resulting in cell death. Important, however, is the demonstration that increasing actin dynamics, either ...
Subjects
Medical Subject Headings: macromolecular substances
free text keywords: Report, actin; senescence; Scp1; apoptosis; ROS, Q, Cell Biology
29 references, page 1 of 2

Ayscough, K.R. 2000. Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton. Curr. Biol. 10:1587-1590. [OpenAIRE]

Belmont, L.D., and D.G. Drubin. 1998. The yeast V159N actin mutant reveals roles for actin dynamics in vivo. J. Cell Biol. 142:1289-1299. [OpenAIRE]

Belmont, L.D., G.M. Patterson, and D.G. Drubin. 1999. New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites. J. Cell Sci. 112:1325-1336.

Boldogh, I.R., N. Vojtov, S. Karmon, and L.A. Pon. 1998. Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p. J. Cell Biol. 141:1371-1381. [OpenAIRE]

Frohlich, K.U., and F. Madeo. 2001. Apoptosis in yeast: a new model for aging research. Exp. Gerontol. 37:27-31.

Gonos, E.S., A. Derventzi, M. Kveiborg, G. Agiostratidou, M. Kassem, B.F.C. Clark, P.S. Jat, and S.I.S. Rattan. 1998. Cloning and identification of genes that associate with mammalian replicative senescence. Exp. Cell Res. 240:66-74.

Goodman, A., B.L. Goode, P. Matsudaira, and G.R. Fink. 2003. The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol. Biol. Cell. 14:2617-2629.

Guthrie, G., and G.R. Fink. 1991. Generation of rho yeast strains. Methods Enzymol. 194:150-151.

Hagan, I.M., and K.R. Ayscough. 2000. Fluorescence microscopy in yeast. In Protein Localization by Fluorescence Microscopy: A Practical Approach. V.J. Allan, editor. Oxford University Press, Oxford, UK. 179-205.

Hampsey, M. 1997. A review of phenotypes in Saccharomyces cerevisiae. Yeast. 13: 1099-1133. [OpenAIRE]

Jazwinski, S.M. 2002. Growing old: metabolic control and yeast aging. Annu. Rev. Microbiol. 56:769-792. [OpenAIRE]

Kim, S., S.G. Hwang, I.C. Kim, and J.S. Chun. 2003. Actin cytoskeletal architecture regulates nitric oxide-induced apoptosis, dedifferentiation and cyclooxygenase-2 expression in articular chondrocytes via mitogen-activated protein kinase and protein kinase C pathways. J. Biol. Chem. 278:42448-42456.

Kusano, H., S. Shimizu, R.C. Koya, H. Fujita, S. Kamada, N. Kuzumaki, and Y. Tsujimoto. 2000. Human gelsolin prevents apoptosis by inhibiting apoptotic mitochondrial changes via closing VDAC. Oncogene. 19:4807-4814. [OpenAIRE]

Laun, P., A. Pichova, F. Madeo, J. Fuchs, A. Ellinger, S. Kohlwein, I. Dawes, and K. Froehlich. 2001. Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis. Mol. Microbiol. 39:1166-1173. [OpenAIRE]

Lin, S.J., P.A. Defossez, and L. Guarente. 2000. Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science. 289:2126-2128.

29 references, page 1 of 2
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