Diagnosis of Barmah Forest virus infection by a nested real-time SYBR green RT-PCR assay.

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Hueston, Linda; Toi, Cheryl S.; Jeoffreys, Neisha; Sorrell, Tania; Gilbert, Gwendolyn;
(2013)
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE,volume 8,issue 7 (issn: 1932-6203, eissn: 1932-6203)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1371/journal.pone.0065197, pmc: PMC3720699
  • Subject: Viral Disease Diagnosis | Travel-Associated Diseases | Research Article | Biology | Diagnostic Medicine | Test Evaluation | Emerging Infectious Diseases | Infectious diseases | Microbiology | Zoonoses | Medicine | Viral Diseases | Barmah Forest virus | Q | R | Emerging Viral Diseases | Virology | Science

Barmah Forest virus (BFV) is a mosquito borne (+) ssRNA alphavirus found only in Australia. It causes rash, myalgia and arthralgia in humans and is usually diagnosed serologically. We developed a real-time PCR assay to detect BFV in an effort to improve diagnosis early ... View more
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