publication . Doctoral thesis . 2002

Peptide-tagged proteins in aqueous two-phase systems

Nilsson, Anna;
Open Access English
  • Published: 01 Jan 2002
  • Publisher: Prof. Folke Tjerneld, Dept. Biochemistry, Lund University
Abstract
This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. However, full effect of the tag as expected from peptide partitioning was not found in the tagged protein. When alkyl-ethylene oxide surfactant was included in a two-polymer system, almost full effect of the tag was obtained. This indicates an improved exposure of the tag to the phase components in the surfactant-containing systems. The most efficient amino acid residue ...
Subjects
free text keywords: Aqueous two-phase system, Biokemi, cutinase, Biochemistry, peptide-tagged protein, metabolism, green fluorescent protein, Biological Sciences, tryptophan
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publication . Doctoral thesis . 2002

Peptide-tagged proteins in aqueous two-phase systems

Nilsson, Anna;