Accumulation, Biotransformation, Histopathology and Paralysis in the Pacific Calico Scallop Argopecten ventricosus by the Paralyzing Toxins of the Dinoflagellate Gymnodinium catenatum

Article, Other literature type English OPEN
Escobedo-Lozano, Amada Y. ; Estrada, Norma ; Ascencio, Felipe ; Contreras, Gerardo ; Alonso-Rodriguez, Rosalba (2012)
  • Publisher: MDPI
  • Journal: Marine Drugs, volume 10, issue 5, pages 1,044-1,065 (issn: 1660-3397, eissn: 1660-3397)
  • Related identifiers: pmc: PMC3397451, doi: 10.3390/md10051044
  • Subject: Gymnodinium catenatum | Argopecten ventricosus | <em>Gymnodinium catenatum</em> | histopathology | paralyzing shellfish poison | paralysis | &lt;em&gt;Gymnodinium catenatum&lt;/em&gt; | Article | QH301-705.5 | <em>Argopecten ventricosus</em> | &lt;em&gt;Argopecten ventricosus&lt;/em&gt; | Biology (General)
    mesheuropmc: animal structures | macromolecular substances | stomatognathic system

The dinoflagellate &lt;em&gt;Gymnodinium catenatum&lt;/em&gt; produces paralyzing shellfish poisons that are consumed and accumulated by bivalves. We performed short-term feeding experiments to examine ingestion, accumulation, biotransformation, histopathology, and paralysis in the juvenile Pacific calico scallop &lt;em&gt;Argopecten ventricosus&lt;/em&gt; that consume this dinoflagellate. Depletion of algal cells was measured in closed systems. Histopathological preparations were microscopically analyzed. Paralysis was observed and the time of recovery recorded. Accumulation and possible biotransformation of toxins were measured by HPLC analysis. Feeding activity in treated scallops showed that scallops produced pseudofeces, ingestion rates decreased at 8 h; approximately 60% of the scallops were paralyzed and melanin production and hemocyte aggregation were observed in several tissues at 15 h. HPLC analysis showed that the only toxins present in the dinoflagellates and scallops were the &lt;em&gt;N&lt;/em&gt;-sulfo-carbamoyl toxins (C1, C2); after hydrolysis, the carbamate toxins (epimers GTX2/3) were present. C1 and C2 toxins were most common in the mantle, followed by the digestive gland and stomach-complex, adductor muscle, kidney and rectum group, and finally, gills. Toxin profiles in scallop tissue were similar to the dinoflagellate; biotransformations were not present in the scallops in this short-term feeding experiment.
  • References (101)
    101 references, page 1 of 11

    1. Graham, H.W. Gymnodinium catenatum, a new dinoflagellate from the Gulf of California. Trans. Am. Microsc. Soc. 1943, 62, 259-261.

    2. Mee, L.D.; Espinoza, M.; Díaz, G. Paralytic shellfish poisoning with a Gymnodinium catenatum red tide of the Pacific Coast of Mexico. Mar. Environ. Res. 1986, 19, 77-92.

    3. Cortés, A.R.; Manrique, F.A.; Luna-Soria, R. Occurrence of red tides in the eastern coast of the Gulf of California. Rev. Lat. Microbiol. 1995, 37, 337-342.

    4. Ochoa, J.L.; Sánchez-Paz, A.; Cruz-Villacorta, A.; Núñez-Vázquez, E.; Sierra-Beltrán, A. Toxic events in the northwest Pacific coastline of Mexico during 1992-1995: Origin and impact. Hydrobiologia 1997, 352, 195-200.

    5. Ochoa, J.L.; Sierra-Beltrán, A.; Alonso-Colmenares, G.; Barradas-Sánchez, H.; Cruz-Villacorta, A.; Nuñez-Vázquez, E.; Sánchez-Paz, A. Biotoxins in the Pacific coast of Mexico. In Mycotoxins and Phycotoxins: Developments in Chemistry, Toxicology, and Food Safety, Proceedings of IX IUPAC (International Union Purity Analytical Chemistry) International Symposium on Mycotoxins and Phycotoxins, Rome, Italy, 27-31 May 1996; Miraglia, M., van Egmond, H.P., Brera, C., Gilbert, J., Eds.; Alaken: Fort Collins, CO, USA, 1998; pp. 441-448.

    6. Alonso-Rodríguez, R.; Ochoa, J.L. Hydrology of winter-spring “red tides” in Bahía de Mazatlán, Sinaloa, México. Harmful Algae 2004, 3, 163-171.

    7. Gárate-Lizárraga, I.; Bustillos-Guzmán, J.J.; Morquecho, L.; Band-Schmidt, C.J.; Alonso-Rodríguez, R.; Erler, K.; Luckas, B.; Reyes-Salinas, A.; Góngora-González, D.T. Comparative paralytic shellfish toxin profiles in the strains of Gymnodinium catenatum Graham from the Gulf of California, Mexico. Mar. Pollut. Bull. 2005, 50, 208-236.

    8. Band-Schmidt, C.J.; Bustillos-Guzmán, J.; Gárate-Lizárraga, I.; Lechuga-Devèze, C.H.; Reinhardt K.; Luckas, B. Paralytic shellfish toxin profile in strains of the dinoflagellate Gymnodinium catenatum Graham and the scallop Argopecten ventricosus G. B. Sowerby II from Bahía Conception, Gulf of California, Mexico. Harmful Algae 2005, 4, 21-31.

    9. Band-Schmidt, C.; Bustillos-Guzmán, J.; Morquecho, L.; Gárate-Lizárraga, I.; Alonso-Rodríguez, R.; Reyes-Salinas, A.; Erler, K.; Luckas, B. Variations of PSP toxin profiles during different growth phases in Gymnodinium catenatum (Dinophyceae) strains isolated from three locations in the Gulf of California, Mexico. J. Phycol. 2006, 42, 757-768.

    10. Cembella, A.D.; Lewis, N.I.; Shumway, S.E. An interspecific comparison of paralytic shellfish poisons in marine bivalves: Anatomical and spatio-temporal variation in toxin composition. J. Shellfish Res. 1993, 12, 389-403.

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