publication . Article . 2012

Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomic 16s rDNA.

Oikonomou, Georgios; Machado, Vinicius Silva; Santisteban, Carlos; Schukken, Ynte Hein; Bicalho, Rodrigo Carvalho;
Open Access English
  • Published: 01 Oct 2012 Journal: PLoS ONE, volume 7, issue 10 (issn: 1932-6203, eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
Abstract
Dairy cow mastitis is an important disease in the dairy industry. Different microbial species have been identified as causative agents in mastitis, and are traditionally diagnosed by bacterial culture. The objective of this study was to use metagenomic pyrosequencing of bacterial 16S rRNA genes to investigate bacterial DNA diversity in milk samples of mastitic and healthy dairy cows and compare the results with those obtained by classical bacterial culture. One hundred and thirty-six milk samples were collected from cows showing signs of mastitis and used for microbiological culture. Additionally, 20 milk samples were collected from healthy quarters. Bacterial D...
Subjects
free text keywords: Microbial Control, Microbial Pathogens, Gram Positive, Research Article, Veterinary Diseases, Agriculture, Bacteriology, Veterinary Microbiology, Escherichia Coli, Biology, Veterinary Science, Emerging Infectious Diseases, Microbiology, Staphylococci, Medicine, Bacterial Pathogens, Gram Negative, Streptococci, Applied Microbiology, Q, Agricultural Biotechnology, Veterinary Bacteriology, R, Bacterial Taxonomy, Science
33 references, page 1 of 3

1 Halasa T, Huijps K, Osteras O, Hogeveen H (2007) Economic e ffects of bovine mastitis and mastitis management: A review. Vet Q 29(1): 18–31.17471788 [OpenAIRE] [PubMed]

2 Taponen S, Salmikivi L, Simojoki H, Koskinen MT, Pyorala S (2009) Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J Dairy Sci 92(6): 2610–2617.19447993 [PubMed]

3 Koskinen MT, Wellenberg GJ, Sampimon OC, Holopainen J, Rothkamp A, et al (2010) Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. J Dairy Sci 93(12): 5707–5715.21094742 [PubMed]

4 Shome BR, Das Mitra S, Bhuvana M, Krithiga N, Velu D, et al (2011) Multiplex PCR assay for species identification of bovine mastitis pathogens. J Appl Microbiol 111(6): 1349–1356.21972842 [PubMed]

5 Kuang Y, Tani K, Synnott AJ, Ohshima K, Higuchi H, et al (2009) Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR–DGGE method. Biochem Eng J 45(1): 76–81.

6 Schwaiger K, Wimmer M, Huber-Schlenstedt R, Fehlings K, Holzel CS, et al (2012) Hot topic: Bovine milk samples yielding negative or nonspecific result s in bacterial culturing–the possible role of PCR-single strand conformation polymorphism in mastitis diagnosis. J Dairy Sci 95(1): 98–101.22192188 [PubMed]

7 Hunt KM, Foster JA, Forney LJ, Schutte UM, Beck DL, et al (2011) Characterization of the diversity and temporal stability of bacterial communities in human milk. PLoS One 6(6): e21313.21695057 [OpenAIRE] [PubMed]

8 Kolbert CP, Persing DH (1999) Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 2(3): 299–305.10383862 [PubMed]

9 Bhatt VD, Ahir VB, Koringa PG, Jakhesara SJ, Ra nk DN, et al (2012) Milk microbiome signatures of subclinical mastitis-affected cattle analysed by shotgun sequencing. J Appl Microbiol 112(4): 639–650.22277077 [PubMed]

10 Sun Y, Cai Y, Mai V, Farmerie W, Yu F, et al (2010) Advanced computational algorithms for microbial community analysis using massive 16S rRNA sequence data. Nucleic Acids Res 38(22): e205.20929878 [OpenAIRE] [PubMed]

11 PyöräläS, Jousimies-Somer H, Mero M (1992) Clinical, bacteriological and therapeutic aspects of bovine mastitis caused by aerobic and anaerobic pathogens. Br Vet J 148(1): 54–62.1551015 [PubMed]

12 Elad D, Friedgut O, Alpert N, Stram Y, Lahav D, et al (2004) Bovine necrotic vulvovaginitis associated with Porphyromonas levii . Emerg Infect Dis 10(3): 505–507.15109423 [OpenAIRE] [PubMed]

13 Santos TM, Gilbert RO, Bicalho RC (2011) Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows. J Dairy Sci 94(1): 291–302.21183039 [PubMed]

14 Bicalho ML, Machado VS, Oikonomou G, Gilbert RO, Bicalho RC (2011) Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Vet Microbiol.

15 Nagaraja TG, Narayanan SK, Stewart GC, Chengappa MM (2005) Fusobacterium necrophorum infections in animals: Pathogenesis and pathogenic mechanisms. Anaerobe 11(4): 239–246.16701574 [PubMed]

33 references, page 1 of 3
Abstract
Dairy cow mastitis is an important disease in the dairy industry. Different microbial species have been identified as causative agents in mastitis, and are traditionally diagnosed by bacterial culture. The objective of this study was to use metagenomic pyrosequencing of bacterial 16S rRNA genes to investigate bacterial DNA diversity in milk samples of mastitic and healthy dairy cows and compare the results with those obtained by classical bacterial culture. One hundred and thirty-six milk samples were collected from cows showing signs of mastitis and used for microbiological culture. Additionally, 20 milk samples were collected from healthy quarters. Bacterial D...
Subjects
free text keywords: Microbial Control, Microbial Pathogens, Gram Positive, Research Article, Veterinary Diseases, Agriculture, Bacteriology, Veterinary Microbiology, Escherichia Coli, Biology, Veterinary Science, Emerging Infectious Diseases, Microbiology, Staphylococci, Medicine, Bacterial Pathogens, Gram Negative, Streptococci, Applied Microbiology, Q, Agricultural Biotechnology, Veterinary Bacteriology, R, Bacterial Taxonomy, Science
33 references, page 1 of 3

1 Halasa T, Huijps K, Osteras O, Hogeveen H (2007) Economic e ffects of bovine mastitis and mastitis management: A review. Vet Q 29(1): 18–31.17471788 [OpenAIRE] [PubMed]

2 Taponen S, Salmikivi L, Simojoki H, Koskinen MT, Pyorala S (2009) Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J Dairy Sci 92(6): 2610–2617.19447993 [PubMed]

3 Koskinen MT, Wellenberg GJ, Sampimon OC, Holopainen J, Rothkamp A, et al (2010) Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. J Dairy Sci 93(12): 5707–5715.21094742 [PubMed]

4 Shome BR, Das Mitra S, Bhuvana M, Krithiga N, Velu D, et al (2011) Multiplex PCR assay for species identification of bovine mastitis pathogens. J Appl Microbiol 111(6): 1349–1356.21972842 [PubMed]

5 Kuang Y, Tani K, Synnott AJ, Ohshima K, Higuchi H, et al (2009) Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR–DGGE method. Biochem Eng J 45(1): 76–81.

6 Schwaiger K, Wimmer M, Huber-Schlenstedt R, Fehlings K, Holzel CS, et al (2012) Hot topic: Bovine milk samples yielding negative or nonspecific result s in bacterial culturing–the possible role of PCR-single strand conformation polymorphism in mastitis diagnosis. J Dairy Sci 95(1): 98–101.22192188 [PubMed]

7 Hunt KM, Foster JA, Forney LJ, Schutte UM, Beck DL, et al (2011) Characterization of the diversity and temporal stability of bacterial communities in human milk. PLoS One 6(6): e21313.21695057 [OpenAIRE] [PubMed]

8 Kolbert CP, Persing DH (1999) Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 2(3): 299–305.10383862 [PubMed]

9 Bhatt VD, Ahir VB, Koringa PG, Jakhesara SJ, Ra nk DN, et al (2012) Milk microbiome signatures of subclinical mastitis-affected cattle analysed by shotgun sequencing. J Appl Microbiol 112(4): 639–650.22277077 [PubMed]

10 Sun Y, Cai Y, Mai V, Farmerie W, Yu F, et al (2010) Advanced computational algorithms for microbial community analysis using massive 16S rRNA sequence data. Nucleic Acids Res 38(22): e205.20929878 [OpenAIRE] [PubMed]

11 PyöräläS, Jousimies-Somer H, Mero M (1992) Clinical, bacteriological and therapeutic aspects of bovine mastitis caused by aerobic and anaerobic pathogens. Br Vet J 148(1): 54–62.1551015 [PubMed]

12 Elad D, Friedgut O, Alpert N, Stram Y, Lahav D, et al (2004) Bovine necrotic vulvovaginitis associated with Porphyromonas levii . Emerg Infect Dis 10(3): 505–507.15109423 [OpenAIRE] [PubMed]

13 Santos TM, Gilbert RO, Bicalho RC (2011) Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows. J Dairy Sci 94(1): 291–302.21183039 [PubMed]

14 Bicalho ML, Machado VS, Oikonomou G, Gilbert RO, Bicalho RC (2011) Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Vet Microbiol.

15 Nagaraja TG, Narayanan SK, Stewart GC, Chengappa MM (2005) Fusobacterium necrophorum infections in animals: Pathogenesis and pathogenic mechanisms. Anaerobe 11(4): 239–246.16701574 [PubMed]

33 references, page 1 of 3
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . 2012

Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomic 16s rDNA.

Oikonomou, Georgios; Machado, Vinicius Silva; Santisteban, Carlos; Schukken, Ynte Hein; Bicalho, Rodrigo Carvalho;