publication . Article . 2016

Ethanolic extract of Schizonepeta tenuifolia attenuates osteoclast formation and activation in vitro and protects against lipopolysaccharide-induced bone loss in vivo

Kim, Ju-Young; Baek, Jong Min; Ahn, Sung-Jun; Cheon, Yoon-Hee; Park, Sun-Hyang; Yang, Miyoung; Choi, Min Kyu; Oh, Jaemin;
Open Access English
  • Published: 01 Aug 2016 Journal: BMC Complementary and Alternative Medicine, volume 16, issue 1 (issn: 1472-6882, eissn: 1472-6882, Copyright policy)
  • Publisher: Springer Nature
Abstract
Background Excessive osteoclast activity is a major cause of metabolic bone disorders, such as osteopenia, rheumatoid arthritis, and osteoporosis. Thus, discovery of agents targeting osteoclast differentiation and bone resorption is important for development of novel treatments for bone diseases. It has been demonstrated that ethanolic extract of schizonepeta tenuifolia (EEST) has potent anti-oxidant and anti-inflammatory activities. However, the beneficial effects of EEST on bone metabolism have not been studied. Therefore, we intend to investigate the effects of EEST on osteoclast differentiation. Methods We examined the effects and mechanisms of action of the...
Subjects
mesheuropmc: musculoskeletal diseases
free text keywords: Research Article, Bone resorption, Osteoclast differentiation, Osteoporosis, Complementary and alternative medicine, Schizonepeta tenuifolia
30 references, page 1 of 2

1. Hadjidakis DJ, Androulakis II. Bone remodeling. Ann N Y Acad Sci. 2006;1092:385-96.

2. Tanaka Y, Nakayamada S, Okada Y. Osteoblasts and osteoclasts in bone remodeling and inflammation. Curr Drug Targets Inflamm Allergy. 2005;4(3):325-8.

3. Hughes DE, Dai A, Tiffee JC, Li HH, Mundy GR, Boyce BF. Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-beta. Nat Med. 1996;2(10):1132-6.

4. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003;423(6937):337-42.

5. Suda T, Kobayashi K, Jimi E, Udagawa N, Takahashi N. The molecular basis of osteoclast differentiation and activation. Novartis Found Symp. 2001;232:235-47.

6. Wada T, Nakashima T, Hiroshi N, Penninger JM. RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol Med. 2006;12(1):17-25.

7. Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, et al. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002;3(6):889-901.

8. Rodan SB, Rodan GA. Integrin function in osteoclasts. J Endocrinol. 1997;154(Suppl):S47-56.

9. Yagi M, Miyamoto T, Sawatani Y, Iwamoto K, Hosogane N, Fujita N, et al. DC-STAMP is essential for cell-cell fusion in osteoclasts and foreign body giant cells. J Exp Med. 2005;202(3):345-51.

10. Goto T, Yamaza T, Tanaka T. Cathepsins in the osteoclast. J Electron Microsc (Tokyo). 2003;52(6):551-8.

11. Suda K, Woo JT, Takami M, Sexton PM, Nagai K. Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1, and RANKL. J Cell Physiol. 2002;190(1):101-8.

12. Sakuma Y, Tanaka K, Suda M, Komatsu Y, Yasoda A, et al. Impaired bone resorption by lipopolysaccharide in vivo in mice deficient in the prostaglandin E receptor EP4 subtype. Infect Immun. 2000;68(12):6819-25. [OpenAIRE]

13. Orcel P, Feuga M, Bielakoff J, De Vernejoul MC. Local bone injections of LPS and M-CSF increase bone resorption by different pathways in vivo in rats. Am J Physiol. 1993;264(3 Pt 1):E391-7.

14. Byun MW. Schizonepeta tenuifolia ethanol extract exerts anti-inflammatory activity through the inhibition of TLR4 signaling in lipopolysaccharidestimulated macrophage cells. J Med Food. 2014;17(3):350-6.

15. Wang BS, Huang GJ, Tai HM, Huang MH. Antioxidant and anti-inflammatory activities of aqueous extracts of Schizonepeta tenuifolia Briq. Food Chem Toxicol. 2012;50(3-4):526-31.

30 references, page 1 of 2
Abstract
Background Excessive osteoclast activity is a major cause of metabolic bone disorders, such as osteopenia, rheumatoid arthritis, and osteoporosis. Thus, discovery of agents targeting osteoclast differentiation and bone resorption is important for development of novel treatments for bone diseases. It has been demonstrated that ethanolic extract of schizonepeta tenuifolia (EEST) has potent anti-oxidant and anti-inflammatory activities. However, the beneficial effects of EEST on bone metabolism have not been studied. Therefore, we intend to investigate the effects of EEST on osteoclast differentiation. Methods We examined the effects and mechanisms of action of the...
Subjects
mesheuropmc: musculoskeletal diseases
free text keywords: Research Article, Bone resorption, Osteoclast differentiation, Osteoporosis, Complementary and alternative medicine, Schizonepeta tenuifolia
30 references, page 1 of 2

1. Hadjidakis DJ, Androulakis II. Bone remodeling. Ann N Y Acad Sci. 2006;1092:385-96.

2. Tanaka Y, Nakayamada S, Okada Y. Osteoblasts and osteoclasts in bone remodeling and inflammation. Curr Drug Targets Inflamm Allergy. 2005;4(3):325-8.

3. Hughes DE, Dai A, Tiffee JC, Li HH, Mundy GR, Boyce BF. Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-beta. Nat Med. 1996;2(10):1132-6.

4. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003;423(6937):337-42.

5. Suda T, Kobayashi K, Jimi E, Udagawa N, Takahashi N. The molecular basis of osteoclast differentiation and activation. Novartis Found Symp. 2001;232:235-47.

6. Wada T, Nakashima T, Hiroshi N, Penninger JM. RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol Med. 2006;12(1):17-25.

7. Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, et al. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002;3(6):889-901.

8. Rodan SB, Rodan GA. Integrin function in osteoclasts. J Endocrinol. 1997;154(Suppl):S47-56.

9. Yagi M, Miyamoto T, Sawatani Y, Iwamoto K, Hosogane N, Fujita N, et al. DC-STAMP is essential for cell-cell fusion in osteoclasts and foreign body giant cells. J Exp Med. 2005;202(3):345-51.

10. Goto T, Yamaza T, Tanaka T. Cathepsins in the osteoclast. J Electron Microsc (Tokyo). 2003;52(6):551-8.

11. Suda K, Woo JT, Takami M, Sexton PM, Nagai K. Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1, and RANKL. J Cell Physiol. 2002;190(1):101-8.

12. Sakuma Y, Tanaka K, Suda M, Komatsu Y, Yasoda A, et al. Impaired bone resorption by lipopolysaccharide in vivo in mice deficient in the prostaglandin E receptor EP4 subtype. Infect Immun. 2000;68(12):6819-25. [OpenAIRE]

13. Orcel P, Feuga M, Bielakoff J, De Vernejoul MC. Local bone injections of LPS and M-CSF increase bone resorption by different pathways in vivo in rats. Am J Physiol. 1993;264(3 Pt 1):E391-7.

14. Byun MW. Schizonepeta tenuifolia ethanol extract exerts anti-inflammatory activity through the inhibition of TLR4 signaling in lipopolysaccharidestimulated macrophage cells. J Med Food. 2014;17(3):350-6.

15. Wang BS, Huang GJ, Tai HM, Huang MH. Antioxidant and anti-inflammatory activities of aqueous extracts of Schizonepeta tenuifolia Briq. Food Chem Toxicol. 2012;50(3-4):526-31.

30 references, page 1 of 2
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publication . Article . 2016

Ethanolic extract of Schizonepeta tenuifolia attenuates osteoclast formation and activation in vitro and protects against lipopolysaccharide-induced bone loss in vivo

Kim, Ju-Young; Baek, Jong Min; Ahn, Sung-Jun; Cheon, Yoon-Hee; Park, Sun-Hyang; Yang, Miyoung; Choi, Min Kyu; Oh, Jaemin;