Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome

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Moreli, Marcos Lázaro; Sousa, Ricardo Luiz Moro de; Figueiredo, Luiz Tadeu Moraes;
(2004)
  • Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
  • Journal: Memórias do Instituto Oswaldo Cruz. (issn: 0074-0276, eissn: 1678-8060)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1590/S0074-02762004000600018
  • Subject: hantavirus cardiopulmonary syndrome | RC955-962 | Microbiology | nested RT-PCR | QR1-502 | N gene of hantavirus | Arctic medicine. Tropical medicine
    mesheuropmc: viruses | virus diseases | animal diseases

We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS) patients from Brazil,... View more