publication . Other literature type . Article . Preprint . 2019

The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation

Julia Batki; Jakob Schnabl; Juncheng Wang; Dominik Handler; Veselin I. Andreev; Christian E. Stieger; Maria Novatchkova; Lisa Lampersberger; Kotryna Kauneckaite; Wei Xie; ...
Open Access
  • Published: 17 Apr 2019
  • Publisher: Springer Science and Business Media LLC
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>The PIWI-interacting RNA (piRNA) pathway protects animal genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA-guided targeting of nuclear PIWI proteins to nascent transposon transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (Silencing Factor interacting Nuclear eXport variant), an interdependent protein complex required for Piwi-mediated co-transcriptional silencing in <jats:italic>Drosophila</jats:italic>. SFiNX consists of Nxf2-Nxt1, a gonad-specific variant of the heterodimeric mRNA export receptor Nxf1-Nxt1, and...
Subjects
Medical Subject Headings: endocrine systemurogenital system
free text keywords: Molecular Biology, Structural Biology, Article, Small RNA, RNA, Piwi-interacting RNA, Transposable element, Heterochromatin, Nuclear export signal, Biology, Cell biology, Messenger RNA, Gene silencing
Funded by
NIH| Pixel Array Detector for X-ray Crystallography
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1S10RR029205-01
  • Funding stream: NATIONAL CENTER FOR RESEARCH RESOURCES
,
FWF| RNA Biology
Project
  • Funder: Austrian Science Fund (FWF) (FWF)
  • Project Code: W 1207
  • Funding stream: Doktoratskollegs (DKs)
,
EC| PIWI-Chrom
Project
PIWI-Chrom
Understanding small RNA-mediated transposon control at the level of chromatin in the animal germline
  • Funder: European Commission (EC)
  • Project Code: 682181
  • Funding stream: H2020 | ERC | ERC-COG
,
NIH| MOUSE GENETICS
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 2P30CA008748-43
  • Funding stream: NATIONAL CANCER INSTITUTE
85 references, page 1 of 6

Adams, P.D., Grosse-Kunstleve, R.W., Hung, L.W., Ioerger, T.R., McCoy, A.J., Moriarty, N.W., Read, R.J., Sacchettini, J.C., Sauter, N.K., and Terwilliger, T.C. (2002). PHENIX: building new software for automated crystallographic structure determination.

Acta crystallographica. Section D, Biological crystallography 58, 1948-1954.

Aibara, S., Katahira, J., Valkov, E., and Stewart, M. (2015). The principal mRNA nuclear export factor NXF1:NXT1 forms a symmetric binding platform that facilitates export of retroviral CTE-RNA. Nucleic acids research 43, 1883-1893. [OpenAIRE]

Andersen, P.R., Tirian, L., Vunjak, M., and Brennecke, J. (2017).

A heterochromatin-dependent transcription machinery drives piRNA expression. Nature 549, 54-59.

Battye, T.G., Kontogiannis, L., Johnson, O., Powell, H.R., and Leslie, A.G. (2011). iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta crystallographica. Section D, Biological crystallography 67, 271- 281. [OpenAIRE]

Braun, I.C., Herold, A., Rode, M., and Izaurralde, E. (2002).

Nuclear export of mRNA by TAP/NXF1 requires two nucleoporinbinding sites but not p15. Molecular and cellular biology 22, 5405- 5418.

Brown, J.B., Boley, N., Eisman, R., May, G.E., Stoiber, M.H., Duff, M.O., Booth, B.W., Wen, J., Park, S., Suzuki, A.M., et al. (2014).

Diversity and dynamics of the Drosophila transcriptome. Nature 512, 393-399.

Caporilli, S., Yu, Y., Jiang, J., and White-Cooper, H. (2013). The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. PLoS genetics 9, e1003526.

Castel, S.E., and Martienssen, R.A. (2013). RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond. Nature reviews. Genetics 14, 100-112. [OpenAIRE]

Combe, C.W., Fischer, L., and Rappsilber, J. (2015). xiNET: crosslink network maps with residue resolution. Mol Cell Proteomics 14, 1137-1147. [OpenAIRE]

Cullen, B.R. (2000). Nuclear RNA export pathways. Molecular and cellular biology 20, 4181-4187.

Czech, B., Munafo, M., Ciabrelli, F., Eastwood, E.L., Fabry, M.H., Kneuss, E., and Hannon, G.J. (2018). piRNA-Guided Genome Defense: From Biogenesis to Silencing. Annual review of genetics 52, 131-157.

85 references, page 1 of 6
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>The PIWI-interacting RNA (piRNA) pathway protects animal genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA-guided targeting of nuclear PIWI proteins to nascent transposon transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (Silencing Factor interacting Nuclear eXport variant), an interdependent protein complex required for Piwi-mediated co-transcriptional silencing in <jats:italic>Drosophila</jats:italic>. SFiNX consists of Nxf2-Nxt1, a gonad-specific variant of the heterodimeric mRNA export receptor Nxf1-Nxt1, and...
Subjects
Medical Subject Headings: endocrine systemurogenital system
free text keywords: Molecular Biology, Structural Biology, Article, Small RNA, RNA, Piwi-interacting RNA, Transposable element, Heterochromatin, Nuclear export signal, Biology, Cell biology, Messenger RNA, Gene silencing
Funded by
NIH| Pixel Array Detector for X-ray Crystallography
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1S10RR029205-01
  • Funding stream: NATIONAL CENTER FOR RESEARCH RESOURCES
,
FWF| RNA Biology
Project
  • Funder: Austrian Science Fund (FWF) (FWF)
  • Project Code: W 1207
  • Funding stream: Doktoratskollegs (DKs)
,
EC| PIWI-Chrom
Project
PIWI-Chrom
Understanding small RNA-mediated transposon control at the level of chromatin in the animal germline
  • Funder: European Commission (EC)
  • Project Code: 682181
  • Funding stream: H2020 | ERC | ERC-COG
,
NIH| MOUSE GENETICS
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 2P30CA008748-43
  • Funding stream: NATIONAL CANCER INSTITUTE
85 references, page 1 of 6

Adams, P.D., Grosse-Kunstleve, R.W., Hung, L.W., Ioerger, T.R., McCoy, A.J., Moriarty, N.W., Read, R.J., Sacchettini, J.C., Sauter, N.K., and Terwilliger, T.C. (2002). PHENIX: building new software for automated crystallographic structure determination.

Acta crystallographica. Section D, Biological crystallography 58, 1948-1954.

Aibara, S., Katahira, J., Valkov, E., and Stewart, M. (2015). The principal mRNA nuclear export factor NXF1:NXT1 forms a symmetric binding platform that facilitates export of retroviral CTE-RNA. Nucleic acids research 43, 1883-1893. [OpenAIRE]

Andersen, P.R., Tirian, L., Vunjak, M., and Brennecke, J. (2017).

A heterochromatin-dependent transcription machinery drives piRNA expression. Nature 549, 54-59.

Battye, T.G., Kontogiannis, L., Johnson, O., Powell, H.R., and Leslie, A.G. (2011). iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta crystallographica. Section D, Biological crystallography 67, 271- 281. [OpenAIRE]

Braun, I.C., Herold, A., Rode, M., and Izaurralde, E. (2002).

Nuclear export of mRNA by TAP/NXF1 requires two nucleoporinbinding sites but not p15. Molecular and cellular biology 22, 5405- 5418.

Brown, J.B., Boley, N., Eisman, R., May, G.E., Stoiber, M.H., Duff, M.O., Booth, B.W., Wen, J., Park, S., Suzuki, A.M., et al. (2014).

Diversity and dynamics of the Drosophila transcriptome. Nature 512, 393-399.

Caporilli, S., Yu, Y., Jiang, J., and White-Cooper, H. (2013). The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. PLoS genetics 9, e1003526.

Castel, S.E., and Martienssen, R.A. (2013). RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond. Nature reviews. Genetics 14, 100-112. [OpenAIRE]

Combe, C.W., Fischer, L., and Rappsilber, J. (2015). xiNET: crosslink network maps with residue resolution. Mol Cell Proteomics 14, 1137-1147. [OpenAIRE]

Cullen, B.R. (2000). Nuclear RNA export pathways. Molecular and cellular biology 20, 4181-4187.

Czech, B., Munafo, M., Ciabrelli, F., Eastwood, E.L., Fabry, M.H., Kneuss, E., and Hannon, G.J. (2018). piRNA-Guided Genome Defense: From Biogenesis to Silencing. Annual review of genetics 52, 131-157.

85 references, page 1 of 6
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