publication . Article . 2013

Proteomic profiling of the hypothalamus in a mouse model of cancer-induced anorexia-cachexia

Ihnatko, Robert; Post, Claes; Blomqvist, Anders;
Open Access English
  • Published: 01 Sep 2013 Journal: British Journal of Cancer, volume 109, issue 7, pages 1,867-1,875 (issn: 0007-0920, eissn: 1532-1827, Copyright policy)
  • Publisher: Nature Publishing Group
Abstract
Background: Anorexia-cachexia is a common and severe cancer-related complication but the underlying mechanisms are largely unknown. Here, using a mouse model for tumour-induced anorexia-cachexia, we screened for proteins that are differentially expressed in the hypothalamus, the brain’s metabolic control centre. Methods: The hypothalamus of tumour-bearing mice with implanted methylcholanthrene-induced sarcoma (MCG 101) displaying anorexia and their sham-implanted pair-fed or free-fed littermates was examined using two-dimensional electrophoresis (2-DE)-based comparative proteomics. Differentially expressed proteins were identified by liquid chromatography-tandem...
Subjects
free text keywords: melanocortin-4 receptor, Molecular Diagnostics, cancer anorexia-cachexia, MCG 101, two-dimensional electrophoresis, Medicin och hälsovetenskap, dynamin 1, Medical and Health Sciences, LC-MS/MS
55 references, page 1 of 4

Argilés, JM, Busquets, S, López-Soriano, FJ. Cytokines as mediators and targets for cancer cachexia. Cancer Treat Res. 2006; 130: 199-217 [PubMed]

Baker, H, Joh, TH, Ruggiero, DA, Reis, DJ. Variations in number of dopamine neurons and tyrosine hydroxylase activity in hypothalamus of two mouse strains. J Neurosci. 1983; 3: 832-843 [OpenAIRE] [PubMed]

Body, JJ. The syndrome of anorexia-cachexia. Curr Opin Oncol. 1999; 11: 255-260 [PubMed]

Bosaeus, I, Daneryd, P, Svanberg, E, Lundholm, K. Dietary intake and resting energy expenditure in relation to weight loss in unselected cancer patients. Int J Cancer. 2001; 93: 380-383 [PubMed]

Butler, AA, Cone, RD. The melanocortin receptors: lessons from knockout models. Neuropeptides. 2002; 36: 77-84 [PubMed]

Carr, KD, Tsimberg, Y, Berman, Y, Yamamoto, N. Evidence of increased dopamine receptor signaling in food-restricted rats. Neuroscience. 2003; 119: 1157-1167 [PubMed]

Chen, AS, Marsh, DJ, Trumbauer, ME, Frazier, EG, Guan, XM, Yu, H, Rosenblum, CI, Vongs, A, Feng, Y, Cao, L, Metzger, JM, Strack, AM, Camacho, RE, Mellin, TN, Nunes, CN, Min, W, Fisher, J, Gopal-Truter, S, MacIntyre, DE, Chen, HY, Van der Ploeg, LH. Inactivation of the mouse melanocortin-3 receptor results in increased fat mass and reduced lean body mass. Nat Genet. 2000a; 26: 97-102 [PubMed]

Chen, AS, Metzger, JM, Trumbauer, ME, Guan, XM, Yu, H, Frazier, EG, Marsh, DJ, Forrest, MJ, Gopal-Truter, S, Fisher, J, Camacho, RE, Strack, AM, Mellin, TN, MacIntyre, DE, Chen, HY, Van der Ploeg, LH. Role of the melanocortin-4 receptor in metabolic rate and food intake in mice. Transgenic Res. 2000b; 9: 145-154 [PubMed]

Chen, S, Liu, F. Interaction of dopamine D1 receptor with N-ethylmaleimide-sensitive factor is important for the membrane localization of the receptor. J Neurosci Res. 2010; 88: 2504-2512 [PubMed]

Cooper, AJ, Plum, F. Biochemistry and physiology of brain ammonia. Physiol Rev. 1987; 67: 440-519 [PubMed]

Cuervo, AM. Autophagy: many paths to the same end. Mol Cell Biochem. 2004; 263: 55-72 [PubMed]

Cummings, DE, Schwartz, MW. Melanocortins and body weight: a tale of two receptors. Nat Genet. 2000; 26: 8-9 [PubMed]

Deans, C, Wigmore, SJ. Systemic inflammation, cachexia and prognosis in patients with cancer. Curr Opin Clin Nutr Metab Care. 2005; 8: 265-269 [PubMed]

Desai, S, Ashby, B. Agonist-induced internalization and mitogen-activated protein kinase activation of the human prostaglandin EP4 receptor. FEBS Lett. 2001; 501: 156-160 [PubMed]

Fearon, K, Arends, J, Baracos, V. Understanding the mechanisms and treatment options in cancer cachexia. Nat Rev Clin Oncol. 2013; 10: 90-99 [PubMed]

55 references, page 1 of 4
Abstract
Background: Anorexia-cachexia is a common and severe cancer-related complication but the underlying mechanisms are largely unknown. Here, using a mouse model for tumour-induced anorexia-cachexia, we screened for proteins that are differentially expressed in the hypothalamus, the brain’s metabolic control centre. Methods: The hypothalamus of tumour-bearing mice with implanted methylcholanthrene-induced sarcoma (MCG 101) displaying anorexia and their sham-implanted pair-fed or free-fed littermates was examined using two-dimensional electrophoresis (2-DE)-based comparative proteomics. Differentially expressed proteins were identified by liquid chromatography-tandem...
Subjects
free text keywords: melanocortin-4 receptor, Molecular Diagnostics, cancer anorexia-cachexia, MCG 101, two-dimensional electrophoresis, Medicin och hälsovetenskap, dynamin 1, Medical and Health Sciences, LC-MS/MS
55 references, page 1 of 4

Argilés, JM, Busquets, S, López-Soriano, FJ. Cytokines as mediators and targets for cancer cachexia. Cancer Treat Res. 2006; 130: 199-217 [PubMed]

Baker, H, Joh, TH, Ruggiero, DA, Reis, DJ. Variations in number of dopamine neurons and tyrosine hydroxylase activity in hypothalamus of two mouse strains. J Neurosci. 1983; 3: 832-843 [OpenAIRE] [PubMed]

Body, JJ. The syndrome of anorexia-cachexia. Curr Opin Oncol. 1999; 11: 255-260 [PubMed]

Bosaeus, I, Daneryd, P, Svanberg, E, Lundholm, K. Dietary intake and resting energy expenditure in relation to weight loss in unselected cancer patients. Int J Cancer. 2001; 93: 380-383 [PubMed]

Butler, AA, Cone, RD. The melanocortin receptors: lessons from knockout models. Neuropeptides. 2002; 36: 77-84 [PubMed]

Carr, KD, Tsimberg, Y, Berman, Y, Yamamoto, N. Evidence of increased dopamine receptor signaling in food-restricted rats. Neuroscience. 2003; 119: 1157-1167 [PubMed]

Chen, AS, Marsh, DJ, Trumbauer, ME, Frazier, EG, Guan, XM, Yu, H, Rosenblum, CI, Vongs, A, Feng, Y, Cao, L, Metzger, JM, Strack, AM, Camacho, RE, Mellin, TN, Nunes, CN, Min, W, Fisher, J, Gopal-Truter, S, MacIntyre, DE, Chen, HY, Van der Ploeg, LH. Inactivation of the mouse melanocortin-3 receptor results in increased fat mass and reduced lean body mass. Nat Genet. 2000a; 26: 97-102 [PubMed]

Chen, AS, Metzger, JM, Trumbauer, ME, Guan, XM, Yu, H, Frazier, EG, Marsh, DJ, Forrest, MJ, Gopal-Truter, S, Fisher, J, Camacho, RE, Strack, AM, Mellin, TN, MacIntyre, DE, Chen, HY, Van der Ploeg, LH. Role of the melanocortin-4 receptor in metabolic rate and food intake in mice. Transgenic Res. 2000b; 9: 145-154 [PubMed]

Chen, S, Liu, F. Interaction of dopamine D1 receptor with N-ethylmaleimide-sensitive factor is important for the membrane localization of the receptor. J Neurosci Res. 2010; 88: 2504-2512 [PubMed]

Cooper, AJ, Plum, F. Biochemistry and physiology of brain ammonia. Physiol Rev. 1987; 67: 440-519 [PubMed]

Cuervo, AM. Autophagy: many paths to the same end. Mol Cell Biochem. 2004; 263: 55-72 [PubMed]

Cummings, DE, Schwartz, MW. Melanocortins and body weight: a tale of two receptors. Nat Genet. 2000; 26: 8-9 [PubMed]

Deans, C, Wigmore, SJ. Systemic inflammation, cachexia and prognosis in patients with cancer. Curr Opin Clin Nutr Metab Care. 2005; 8: 265-269 [PubMed]

Desai, S, Ashby, B. Agonist-induced internalization and mitogen-activated protein kinase activation of the human prostaglandin EP4 receptor. FEBS Lett. 2001; 501: 156-160 [PubMed]

Fearon, K, Arends, J, Baracos, V. Understanding the mechanisms and treatment options in cancer cachexia. Nat Rev Clin Oncol. 2013; 10: 90-99 [PubMed]

55 references, page 1 of 4
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publication . Article . 2013

Proteomic profiling of the hypothalamus in a mouse model of cancer-induced anorexia-cachexia

Ihnatko, Robert; Post, Claes; Blomqvist, Anders;