Cryopreservation-related loss of antigen-specific IFNγ producing CD4+ T-cells can skew immunogenicity data in vaccine trials: lessons from a Malaria vaccine trial substudy

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Ford, T; Wenden, C; Mbekeani, A; Dally, L; Cox, JH; Morin, M; Winstone, N; Hill, AVS; Gilmour, J; Ewer, K;
  • Publisher: Elsevier Science
  • Journal: Vaccine,volume 35,issue 15,pages1,898-1,906 (issn: 1873-2518 1873-2518, eissn: 1873-2518)
  • Related identifiers: pmc: PMC5387668, doi: 10.1016/j.vaccine.2017.02.038
  • Subject: CSP | Immunology and Microbiology(all) | Infectious Diseases | Malaria | Article | Cryopreservation | ChAd63 | veterinary(all) | Molecular Medicine | MVA | TRAP | PBMC | Public Health, Environmental and Occupational Health

Ex vivo functional immunoassays such as ELISpot and intracellular cytokine staining (ICS) by flow cytometry are crucial tools in vaccine development both in the identification of novel immunogenic targets and in the immunological assessment of samples from clinical tria... View more
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