publication . Article . 1999

Production of Monoclonal Antibodies specific for Progesterone

YÜCEL, Fatıma;
Open Access Turkish
  • Published: 01 Apr 1999 Journal: issn: 1300-0152, eissn: 1303-6092, Copyright policy
  • Publisher: TUBITAK
Abstract
Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were charact...
Subjects
free text keywords: Immunodiagnosis, monoclonal antibody, progesterone, ELISA
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publication . Article . 1999

Production of Monoclonal Antibodies specific for Progesterone

YÜCEL, Fatıma;