Effects of Depilation-Induced Skin Pigmentation and Diet-Induced Fluorescence on In Vivo Fluorescence Imaging

Article English OPEN
Kwon, Sunkuk ; Sevick-Muraca, Eva M. (2017)
  • Publisher: Hindawi
  • Journal: Contrast Media & Molecular Imaging (issn: 1555-4309, vol: 2,017)
  • Related identifiers: pmc: PMC5612701, doi: 10.1155/2017/7659242
  • Subject: Research Article | R855-855.5 | Article Subject | Medical technology
    mesheuropmc: sense organs

Near-infrared fluorescence imaging (NIRFI) and far-red fluorescence imaging (FRFI) were used to investigate effects of depilation-induced skin pigmentation and diet-induced background fluorescence on fluorescent signal amplitude and lymphatic contraction frequency in C57BL6 mice. Far-red fluorescent signal amplitude, but not frequency, was affected by diet-induced fluorescence, which was removed by feeding the mice an alfalfa-free diet, and skin pigmentation further impacted the amplitude measurement. NIRFI showed minimal background fluorescence; however, skin pigmentation reduced the amplitude of fluorescent signal changes. Therefore, these effects should be taken into account when imaging mice with different states of skin pigmentation and diet-induced background fluorescence in vivo.
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