Nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis

Article, Other ORP type, Unknown English OPEN
Macedo, Maíra Bidart ; Groll, Andrea Von ; Fissette, Krista ; Palomino, Juan Carlos ; da Silva, Pedro Eduardo Almeida ; Martin, Anandi (2012)
  • Publisher: Sociedade Brasileira de Microbiologia
  • Journal: Brazilian Journal of Microbiology, volume 43, issue 3, pages 981-983 (issn: 1517-8382, eissn: 1678-4405)
  • Related identifiers: doi: 10.1590/S1517-83822012000300019, doi: 10.1590/S1517-838220120003000019, pmc: PMC3768877
  • Subject: Multidrug resistance | Detection | nitrate reductase assay | tuberculosis | Microbiology | QR1-502 | Mycobacterium tuberculosis | Short Communication | Strains | multidrug resistant, sodium nitrate | Bacterial diseases | Medical Microbiology | Laboratory diagnosis | Rapid diagnostic tests | Specificity | Sensitivity | Laboratory techniques and procedures | sodium nitrate | multidrug resistant

We validated the nitrate reductase assay (NRA) for the detection of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) using sodium nitrate (NaNO3) in replacement of potassium nitrate (KNO3) as nitrate source. NaNO3 is cheaper than KNO3 and has no restriction on use which facilitates the implementation of NRA to detect MDR-TB.