Anti-Inflammatory Effects of Lactobacillus Rahmnosus and Bifidobacterium Breve on Cigarette Smoke Activated Human Macrophages

Article, Unknown English OPEN
Mortaz, Esmaeil ; Adcock, Ian M. ; Ricciardolo, Fabio L. M. ; Varahram, Mohammad ; Jamaati, Hamidreza ; Velayati, Ali Akbar ; Folkerts, Gert ; Garssen, Johan (2015)
  • Publisher: Public Library of Science
  • Journal: PLoS ONE, volume 10, issue 8 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: doi: 10.1371/journal.pone.0136455, pmc: PMC4552661
  • Subject: OBSTRUCTIVE PULMONARY-DISEASE | MD Multidisciplinary | Research Article | CHRONIC ASTHMA | RELEASE | INFLAMMATORY-BOWEL-DISEASE | ALVEOLAR MACROPHAGES | Medicine | Multidisciplinary Sciences | MURINE MODEL | EPITHELIAL-CELLS | COPD | Q | R | Science & Technology | Science | General Science & Technology | Science & Technology - Other Topics | NEUTROPHILS | PROBIOTICS
    mesheuropmc: food and beverages

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a major global health problem with cigarette smoke (CS) as the main risk factor for its development. Airway inflammation in COPD involves the increased expression of inflammatory mediators such as CXCL-8 and IL-1β which are important mediators for neutrophil recruitment. Macrophages are an important source of these mediators in COPD. Lactobacillus rhamnosus (L. rhamnosus) and Befidobacterium breve (B. breve) attenuate the development of 'allergic asthma' in animals but their effects in COPD are unknown. OBJECTIVE: To determine the anti-inflammatory effects of L. rhamnosus and B. breve on CS and Toll-like receptor (TLR) activation. DESIGN: We stimulated the human macrophage cell line THP-1 with CS extract in the presence and absence of L. rhamnosus and B. breve and measured the expression and release of inflammatory mediators by RT-qPCR and ELISA respectively. An activity assay and Western blotting were used to examine NF-κB activation. RESULTS: Both L. rhamnosus and B. breve were efficiently phagocytized by human macrophages. L. rhamnosus and B. breve significantly suppressed the ability of CS to induce the expression of IL-1β, IL-6, IL-10, IL-23, TNFα, CXCL-8 and HMGB1 release (all p<0.05) in human THP-1 macrophages. Similar suppression of TLR4- and TLR9-induced CXCL8 expression was also observed (p<0.05). The effect of L. rhamnosus and B. breve on inflammatory mediator release was associated with the suppression of CS-induced NF-κB activation (p<0.05). CONCLUSIONS: This data indicate that these probiotics may be useful anti-inflammatory agents in CS-associated disease such as COPD.
  • References (59)
    59 references, page 1 of 6

    1. Rabe KF, Hurd S, Anzueto A, Barnes PJ, Buist SA, Calverley P, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. American Journal of Respiratory and Critical Care Medicine. 2007; 176:532-555. PMID: 17507545

    2. Barnes PJ “Neutrophils find smoke attractive,” Science. 2010; 210:40-41.

    3. Adcock IM, Caramori G, and Barnes PJ Chronic obstructive pulmonary disease and lung cancer: new molecular insights. Respiration. 2011; 81: 265-268. doi: 10.1159/000324601 PMID: 21430413

    4. Rennard SI and Vestbo J COPD: the dangerous underestimate of 15%. The Lancet. 2006; 367:1216- 1219.

    5. Cosio MG, Saetta M, and Agusti A. Immunologic aspects of chronic obstructive pulmonary disease. The New England Journal of Medicine. 2009; 360:2396-2454.

    6. Hogg JC, Chu F, Utokaparch S., Woods R, Elliott WM, Buzatu L, et al., “The nature of small airway obstruction in chronic obstructive pulmonary disease,” The New England Journal of Medicine. 2004; 350:2645-2653. PMID: 15215480

    7. Pons AR, Noguera A, Blanquer D, Sauleda J, Pons J, Agustí AG Phenotypic characterisation of alveolar macrophages and peripheral blood monocytes in COPD. Eur Respir J. 2005; 25:647-52. PMID: 15802338

    8. Barnes PJ, Hansel TT. Prospects for new drugs for chronic obstructive pulmonary disease. Lancet. 2004; 364: 985-96. PMID: 15364192

    9. Tzanakis NE, Tsiligianni IG, and Siafakas NM. Pulmonary involvement and allergic disorders in inflammatory bowel disease. World Journal of Gastroenterology. 2010; 16:299-305. PMID: 20082474

    10. Black H, Mendoza M, and Murin S. “Thoracic manifestations of inflammatory bowel disease. Chest. 2007; 131:524-532. PMID: 17296657

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